Extraction * 6 Precipitation and differential solubilization * 7 Ultracentrifugation * 8 Chromatographic methods * 8.1 Size exclusion chromatography * 8.2 Separation based on charge or hydrophobicity * 8.3 Ion exchange chromatography * 8.4 Affinity chromatography * 8.4.1 Metal binding * 8.4.2 Immunoaffinity chromatography * 8.4.3 Purification of a tagged protein * 8.5 HPLC * 9 Concentration of the purified protein * 9.1 Lyophilization
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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Photosynthesis Lab Report Introduction The lights are essential for photosynthesis and it plays a major role. All the food we eat and all the fossil fuel we use is a product of photosynthesis‚ which is the process that converts energy in sunlight to chemical forms of energy that can be used by biological systems. Photosynthesis is carried out by many different organisms but usually by plants and algae. All these organisms convert CO2 (carbon dioxide)
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IS MATTER AROUND US PURE matter Pure substance Element Compound Mixture Homogenous Heterogeneous colloid Suspension True solution MATTER Everything in the Universe is made up of MATTER PURE SUBSTANCE The substance which contains same kind of Atom or Molecule i] element A pure substance which contain same kind of Atom ii] compound A pure substance which contain same kind of Atom or Molecule but made up of different Atoms through a Chemical bound Mixture Mixture are constitutor
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examination of color‚ texture‚ patterns‚ twist‚ cross-sectional‚ and morphological characteristics. (Saferstein‚ 2015) Most fiber evidence lies in criminalist’s ability to trace their origin. Fabrics have made it fiber evidential value‚ due to the dye in cotton. Williams’s home‚ car‚ and a yellowish-green blanket found underneath Williams’s bed during the search provided various fibers similar to those found on the victims’ bodies. These fibers were yellowish-green nylon and violet acetate. A criminalist
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Preparation of Dibenzalacetone by the Aldol condensation David o Neill Date of experiment: 14/12/2011 Apparatus Steam bath‚ ice bath‚ Buchner funnel‚ beaker‚ conical flask‚ filter paper‚ TLC apparatus‚ Melting point apparatus Materials / chemicals Benzaldehyde‚ acetone‚ ethanolic sodium hydroxide‚ ethanol Introduction The synthesis of dibenzalacetone is formed from an Aldol condensation reaction. An Aldol condensation reaction is a very effective way of forming a carbon – carbon bond reaction‚ in
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C hapter 2 IS MATTER AROUND US PURE How do we judge whether milk‚ ghee‚ butter‚ salt‚ spices‚ mineral water or juice that we buy from the market are pure? physical process of evaporation. However‚ sodium chloride is itself a substance and cannot be separated by physical process into its chemical constituents. Similarly‚ sugar is a substance because it contains only one kind of pure matter and its composition is the same throughout. Soft drink and soil are not single substances. Whatever the source
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Technology IX Science & Technology Information Center Master List of Investigatory Project Titles 01 Investigatory projects I. Pyrolisis of plastic wastes materials for the production of plywood substitute II. Marang (Artocarpus ordorotissimus) peelings as hardiflex – like III. Ethyl alcohol from ripe banana peelings IV. Rat killer extract from tuble roots V. Fuel briquettes from dried banana leaves and waste papers VI. Coconut sheath substitute of abaca fiber VII. Commercial glue
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Perform an experiment 9 safety Concerns 11 science lab safety reinforcement agreement experiments 14 laboratory techniques and measurements 90 Caloric Content of Food 111 le Chatelier’s Principle 145 Chromatography of Food Dyes appendix 199 laboratory equipment and techniques 204 material safety Data sheets 206 how to Write lab notes and lab reports 212 laboratory Drawings www.HOLscience.com 3 ©Hands-On Labs‚ Inc. www.HOLscience
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the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML --) . Ni2+ agarose affinity chromatography was used to purify the crude extract and different
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