L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally Affinity Chromatography on a Cibacron blue Sepharose stationary
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Partners Lab: Nicole Mintz‚ Bayla Kronman and Dvora Feinblum ABSTRACT This experiment will enable us to separate the pigments of a plant by using the method of paper chromatography. Thanks to this technique the pigments were painted on the strip of paper‚ and we were able to calculate the distance each of them went up: the Rf value. We saw that carotene went the farther up and that chlorophyll ’a’ stayed next to the bottom
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Biochemistry Exam I Terminology Boiling point is the temperature at which the vapor pressure of the liquid equals the pressure surrounding the liquid. At this temperature the liquid phase changes into the gaseous phase (also a colligative property) Melting point is the temperature at which the solid and liquid phases are in equilibrium. At this temperature the solid state changes into a liquid phase (also a colligative property) Specific heat is the amount of energy (in joules or calories) needed
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and Phenylalanine (not in sequence). We compare the Rf values obtained from unknown and compare it with the standards. The amino acid identity is the one that has the lowest absolute value of difference with the unknown. In using the thin layer chromatography in the experiment‚ Sanger’s method was also incorporated to determine the proper sequence of the dipeptide. It is a colorimetric method that uses 1- fluoro- 2‚4- dinitrobenzene (FDNB) and reacts with free amino ‚ imidazole and phenolic groups
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To separate acetaminophen‚ aspirin and caffeine from painkilling drugs. 2.To determine the melting points of acetaminophen‚ aspirin and caffeine 3.To identify the separated components(acetaminophen‚ aspirin and caffeine) by thin-layer chromatography Apparatus and Reagents Used : 1. 4:1 (v/v) 1-Butyl ethanoate/ethanoic acid 2. 3M Sodium hydroxide solution 3. 3M Hydrochloric acid 4. Iodine 5. Dichloromethane 6. Ethanol 7. Anhydrous MgSO4 8. Mortar and pestle 9. Beakers 10. Capillary
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Whiting was questioned about the disappearance of Payne‚ which had taken place about 8 km (5 miles) from Whiting’s place of residence. Whiting was routinely questioned as he had been placed on the Sex Offenders Register. The officers left Whiting‚ but were suspicious of his lack of concern for Payne‚ something that some of the worst offenders had shown when questioned in connection with Payne’s disappearance.[citation needed] When Whiting re-appeared soon after he attempted to drive away in his van
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Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound‚ which is identified by thin layer chromatography (TLC). Eugenol is widely used in dentistry‚ due to its analgesic‚ antiseptic balsamic qualities. It is ideal for curative for pulp hyperemia (the soft‚ sensitive substance underneath the enamel and dentine of a tooth) and pulp its. In dentistry eugenol is also used to
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Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance purified
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Chemistry IGCSE Paper 6 revision guide You can change the temperature and concentration used (not both at the same time though) You need to keep the diameter of the conical flask the same‚ if it is thinner then the cross will disappear sooner‚ the cross also has to be the same‚ volumes too (This set up is mostly if you want to test the gases‚ otherwise you can use the standard set up) The limestone cycle: 1. Burn the limestone
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An electrophilic aromatic substitution reaction was performed on bromobenzene with nitric acid producing both 2-bromonitrobenzene and 4-bromonitrobenzene. Products of the reaction were purified through multiple recrystallizations and column chromatography creating multiple crops of a yellow powder. The percent yield of products was determined to be 51%. The melting point of Crop 1 was found to be 110-115 °C‚ and Crop 2 was found to be 37-90 °C indicating impurity in both crops. TLC analysis shows
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