antioxidant. Lycopene is found in various fruits and vegetables. The purpose of this experiment is the isolation of Lycopene from tomato paste. This is done using liquid/solid extraction and chromatography. Once the Lycopene is isolated‚ IR spectroscopy will determine its percentage actually obtained by chromatography. Procedure: A massed sample of 1.012g of tomato paste was placed in a 125mL Erlenmeyer flask. To the flask‚ 5mL of 50:50 hexane-acetone was added into the flask. After the 50:50
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investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary phase – the part of the apparatus that does not move with the sample) and chromatography solvent that was 90% petroleum ether and 10% propanol (mobile
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occurrence of an E1 mechanism in an alcohol dehydration reaction. Specifically this experiment involved the dehydration of 2-methylcylcohexanol by heating this alcohol in the presence of phosphoric acid and then to based on the results of the gas chromatography of the product to determine whether the alcohol exhibited the Evelyn Effect. The reaction taking place for both the Cis and the Trans isomers for Methylcyclohexanol are as follows: E1 Mechanism for Cis Methylcyclohexanol E1 Mechanism for
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Separating Ferrocene and Acetylferrocene by Adsorption Chromatography Separating Ferrocene and Acetylferrocene by Adsorption Chromatography Dry Pack Method Leah Monroe February 27‚ 2003 Organic Chemistry Lab II Experiment performed on February 18 and 20‚ 2003 Abstract: Adsorption column chromatography is one way to separate compounds out of a mixture. In this technique‚ a solid stationary phase called the adsorbent is packed in a glass column and an eluent‚ which is the mobile phase‚ moves
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Filtration is commonly the mechanical or physical operation which is used for the separation of solids from fluids (liquids or gases) by interposing a medium through which only the fluid can pass. Oversize solids in the fluid are retained‚ but the separation is not complete; solids will be contaminated with some fluid and filtrate will contain fine particles (depending on the pore size and filter thickness). Filtration is also used to describe some biological processes‚ especially in water treatment and sewage
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Fluorenone (2) was separated by column chromatography. Chemical Equation: Fluorene (1) Fluorenone (2) Mp 114C mp 83C MW 166.22 MW 180.21 Introduction: Column chromatography was invented by Tswett in 1906 so that his study of the chemistry of chlorophyll could be facilitated. Tswett made significant advances in the method and demonstrated its applicability to the separation of many different kinds of mixtures. Column chromatography rapidly found application in all areas
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Contents Title Page No. 1.0 Introduction 1 2.0 Sample Treatment 3 3.0 Extraction 4 4.1 Solid-Phase Extraction 4.0 Separation 6 5.2 High Performance Liquid Chromatography (HPLC) 5.3 Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein 18
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Performance Liquid Chromatography but is also referred to as High Pressure Liquid Chromatography. It is used for separating mixtures either to analyse the mixture or to separate a required product from others in a reaction mixture. It can also be used to find the relative amounts of different components in a mixture. HPLC works along the same lines as paper chromatography. In paper chromatography a liquid (mobile phase) moves past a solid (stationary phase). In paper chromatography the stationary phase
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silica gel‚ which acts as a Lewis acid catalyst to facilitate the reaction. The reaction forms a porphyrinogen‚ which is then oxidized to the porphyrin product by atmospheric oxygen. Column chromatography is performed for the isolation and purification of tetraphenlyporphin‚ and the thin layer chromatography for analysis.It was concluded that the renention factor(Rf) of the 5‚10‚15‚20- Tetraphenylporphyrin with a percent yield of 61% Introduction: In this experiment 5‚10‚15‚20‚ tetraphenylporphyrin
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Lab 5 Column Chromatography: Isolation of Lycopene from Tomato Paste Reading: Zubrick‚ pages 79-82‚ 127-130‚ 138-139‚ 141-143‚ and 235-240 Pre-lab: look up the structure of lycopene. Introduction: Lycopene is the red pigment in ripe tomatoes and‚ as an antioxidant‚ helps to fight certain cancers. In this lab you will isolate lycopene from tomato paste. To do this you will first extract carotenoid pigments from the paste and then use column chromatography to isolate the lycopene from
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