CHM130 Lab 9 Chromatography Name: Karlee Rose A. Data Table (12 points) Paper # Color Source Solvent Distance Component Moves Distance Solvent Moves Rf value 1 Yellow M&M Candies 0.1% Salt Solution 28.88mm 42mm 0.69 2 Yellow Reese’s Pieces 0.1% Salt Solution 16.95mm 32mm 0.53 3 Purple Grape Soda 0.1% Salt Solution 32.15mm 51mm 0.63 4 Purple Grape Koolaid 0.1% Salt Solution 12.12mm 31mm 0.39 5 Red Easter Egg Dye 0.1% Salt Solution 1.18mm 7mm 0.17 6 Red Dry Erase Marker
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C hapter 2 IS MATTER AROUND US PURE How do we judge whether milk‚ ghee‚ butter‚ salt‚ spices‚ mineral water or juice that we buy from the market are pure? physical process of evaporation. However‚ sodium chloride is itself a substance and cannot be separated by physical process into its chemical constituents. Similarly‚ sugar is a substance because it contains only one kind of pure matter and its composition is the same throughout. Soft drink and soil are not single substances. Whatever the source
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best. The idea is to be able to isolate the protien that is in interest from the protiens that are not needed. Below are several chromatography techniques that are used to analyze proteins. Size exclusion chromatography (SEC) In this type of technique the protein is separated according to the size of the protein. The matrixes used in size exclusion chromatography have a range of beads with different pore sizes (H. Dai). The seperation of the protein is dependent on how the protein can enter
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Analysis of Ethanol in Moonshine | Using the GC-MS | Jennifer Greene | ------------------------------------------------- 4 May 2012 ------------------------------------------------- 4 May 2012 Introduction: In this experiment gas chromatography is applied to separate the water-ethanol mixture. This method is often used in the determination of alcohol in blood or urine. One obvious application is when law enforcement agencies need to determine whether or not someone is inebriated. In
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at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion. This means that the affinity chromatography removed a big percentage of contaminating proteins. However‚ removing this huge amount of protein left us with a small amount of LDH
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally Affinity Chromatography on a Cibacron blue Sepharose stationary
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Biochemistry Exam I Terminology Boiling point is the temperature at which the vapor pressure of the liquid equals the pressure surrounding the liquid. At this temperature the liquid phase changes into the gaseous phase (also a colligative property) Melting point is the temperature at which the solid and liquid phases are in equilibrium. At this temperature the solid state changes into a liquid phase (also a colligative property) Specific heat is the amount of energy (in joules or calories) needed
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and Phenylalanine (not in sequence). We compare the Rf values obtained from unknown and compare it with the standards. The amino acid identity is the one that has the lowest absolute value of difference with the unknown. In using the thin layer chromatography in the experiment‚ Sanger’s method was also incorporated to determine the proper sequence of the dipeptide. It is a colorimetric method that uses 1- fluoro- 2‚4- dinitrobenzene (FDNB) and reacts with free amino ‚ imidazole and phenolic groups
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To separate acetaminophen‚ aspirin and caffeine from painkilling drugs. 2.To determine the melting points of acetaminophen‚ aspirin and caffeine 3.To identify the separated components(acetaminophen‚ aspirin and caffeine) by thin-layer chromatography Apparatus and Reagents Used : 1. 4:1 (v/v) 1-Butyl ethanoate/ethanoic acid 2. 3M Sodium hydroxide solution 3. 3M Hydrochloric acid 4. Iodine 5. Dichloromethane 6. Ethanol 7. Anhydrous MgSO4 8. Mortar and pestle 9. Beakers 10. Capillary
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