Chemistry 100 Experiment #2: Chromatography of Food Dyes Name: Cassandra DeVaux Date: April 17‚ 2014 Purpose: To learn how mixtures of compounds can be separated and what food dyes are found in certain foods Introduction: In this exercise we will separate food dyes from a variety of sources. We will first gather data on known dyes where we know how many substances make up the dye. Then we will collect data on some samples where we do not know the make-up. We will compare our
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ORGANIC CHEMISTRY 1 LAB EXPERIMENT NO. 1 to 6 DISCUSSION EXPERIMENT NO. 1: Mel=ng Points And Boiling Points Of Organic Compounds Mel=ng Point -‐ temperature at which the liquid and solid are in equilibrium at a pressure of 1 atm Mel=ng Point Range -‐ determines the purity of a solid sample -‐ temp at
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protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing and pooling. Stage 4 involves the Solvent/Detergent (S/D) viral inactivation which is the first dedicated viral clearance step. At Stage 5‚ the product undergoes cation exchange chromatography which aids in removing the S/D reagents
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Thin-Layer Chromatography of Steroid Hormones Physiology Report 4.2 March 7th‚ 2012 By: Taryn Westerman Donald Fru Gloria Villarreal Julian Garcia Objective The purpose of this experiment was to test the solubility of various steroids in order to obtain more information about their structure. An unknown was also given to be included in the experiment. The test that was performed was thin-layer chromatography which required a glass plate. The known steroids used in this experiment were
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due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow for a purer separation than TLC plates because it has a more polar surface than silica gel does. The less polar dye moves first because it is not as soluble in the stationary
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High-performance liquid chromatography (formerly referred to as high-pressure liquid chromatography)‚ HPLC‚ is a chromatographic technique used to separate the components in a mixture‚ to identify each component‚ and to quantify each component. HPLC is considered an instrumental technique of analytical chemistry (as opposed to a gravimetric technique). In general‚ the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent.
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SAMPLE PREPARATION METHODS FOR LIQUID SAMPLES Table of Contents SAMPLE PREPARATON Analytical procedures consist of numerous stages‚ the most important of which is the collection of a sample and its preparation for analysis as samples are usually not in a suitable form for direct introduction into analytical instruments (Tankiewicz et al.‚ 2011). Sample preparation can be thought of as any treatment that the sample is subjected to following its collection‚ prior to its analysis
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HPLC Theory: System Suitability Parameters High performance liquid chromatography is defined as a separation of mixtures of compounds due to differences in their distribution equilibrium between two phases‚ the stationary phase packed inside columns and the mobile phase‚ delivered through the columns by high pressure pumps. Components whose distribution into the stationary phase is higher‚ are retained longer‚ and get separated from those with lower distribution into the stationary phase. The
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The knowledge of chemical analysis today has become important not only for scientists in their research but in fact bears influence in our daily routine as well. To recognize and classify these uses‚ we have to first understand‚ what is chemical analysis?Chemical AnalysisChemical Analysis is a body of procedures and techniques used to identify and quantify the chemical composition of a sample of substance.1 It may be said as a collection of all the techniques required to obtain any chemical information
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Current Regulations for Standardization of Crude Drugs In India a great deal of bulk knowledge exists among ordinary people about the traditional use of herbal medicine. It is difficult to quantify the market size of the traditional Indian system. Since most practitioners formulate and dispense their own recipes. The present annual turnover of product manufactured by large companies is estimated at approximately US $ 300 million compared to a turnover of approximately US $ 2.5 billions for modern
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