pH and Chromatography Lab Report Kevin Rivera Biology Mr. Langley 2C 10/7/13 Introduction In this lab‚ of pH and Chromatography‚ in the pH aspect of the lab we are trying to figure out the pH level of certain chemicals by writing down of known solutions to find the type of unkown solution using pH standards. In the Chromatography‚ we are trying to separate the chemicals using water and a piece of paper. Methodology Materials: Safety Goggles pH indicator & pH indicator key
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of our purer LDH sample that we were unable to collect and use. Another thing to note is that our values for the affinity chromatography step were almost the same as the amount for the size exclusion chromatography step. The total activity was 96.50U and the percent yield was 29.76% for the size exclusion. The lack of purity and low recovery of our size exclusion chromatography could be due to the column beads not being packed and settled before we ran our sample through it‚ since the column leaked
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the sample. Uses for Chromatography: *Pharmaceutical company - can determine how much chemical is in a new product or food. *Hospital- Can detect blood or alcohol levels in a patient’s blood. *Law enforcement- Can compare a sample found at a crime from the suspect. *Environment agency- Can determine the levels of pollutant in the water supply. *Manufacturing Plant- Can purify a chemical needed to make a product. Chromatography is very important
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First‚ the amount of total protein removed by the Affinity Chromatography is difficult to quantify because there appears to be an error in the analysis data for 65% cut precipitate. The protein from the previous purification step limits the amount of protein that can be recovered by the subsequent purification step. In this case‚ the total protein recovered by 65% cut was 159 mg‚ but Affinity Chromatography recovered 350 mg of protein. Purification steps cannot gain protein unless
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notes on the relationship between the structure‚ properties and functions of glycogen and cellulose. 10) Write notes on the 3 main types of column chromatography (not HPLC)‚ explaining their mode of action and principal uses. 11) Write notes on TWO of the following chromatographic techniques: Thin layer chromatography‚ gel (size exclusion) chromatography‚ ion exchange
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Extraction * 6 Precipitation and differential solubilization * 7 Ultracentrifugation * 8 Chromatographic methods * 8.1 Size exclusion chromatography * 8.2 Separation based on charge or hydrophobicity * 8.3 Ion exchange chromatography * 8.4 Affinity chromatography * 8.4.1 Metal binding * 8.4.2 Immunoaffinity chromatography * 8.4.3 Purification of a tagged protein * 8.5 HPLC * 9 Concentration of the purified protein * 9.1 Lyophilization
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LEE ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 88‚ NO. 5‚ 2005 1269 DIETARY SUPPLEMENTS Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices‚ Beverages‚ Natural Colorants‚ and Wines by the pH Differential Method: Collaborative Study JUNGMIN LEE U.S. Department of Agriculture‚ Agricultural Research Service‚ Pacific West Area (PWA)‚ Horticultural Crops Research Laboratory Worksite‚ 29603 University of Idaho Ln‚ Parma‚ ID 83660 ROBERT W. DURST and RONALD E. WROLSTAD Oregon
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IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities and adsorption
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solutions: Lab report: outline Ink chromatography Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. It is used to separate substances. Examples of chromatography are finger-printing‚ medicines‚ and separating/ testing histamines. http://www.biotecharticles.com/Agriculture-Article/Applications-of-Chromatography-1299.html The purpose of this lab
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List of Chemistry Terms used in daily life α (alpha)‚ β (beta) α- (β-‚ γ-) ray spectrometer α-addition (alpha-addition) α-cleavage (alpha-cleavage) α-decay (alpha-decay) α-effect α-elimination α-expulsion in photochemistry α-oxo carbenes α-particle (alpha-particle) ’A’ value -factorquantity ab initio quantum mechanical methods abatement in atmospheric chemistry abeo- abiological abiotic abiotic transformation absolute activation analysis absolute activity‚ quantity absolute
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