Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
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1. Convert %T to absorbance and prepare a Beer’s Law plot using this data. Concentration of Various Samples Sample Identification Code Concentration of M (mol/L) %T A = 2 - log(%T) Q5000 4.00 x 10-4 17.9 Q5001 3.20 x 10-4 25.0 Q5002 2.40 x 10-4 35.7 Q5003 1.60 x 10-4 50.2 Q5004 8.000 x 10-5 70.8 Questions from Procedures 3. What is the concentration of M in these samples? Concentration of M Sample Identification Code %T A = 2
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Floating Kollidon SR (KSR) matrix tablets containing famotidine was developed to increase gastric residence time and bioavailability after oral administration. Total six formulations were formulated by direct compression technique using varying concentrations of Kollidon SR (floating agent). The formulations were evaluated for their drug content‚ hardness‚ friability‚ buoyancy lag time‚ total floating time‚ swelling index and invitro drug release. All formulations possessed good floating properties
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solution. From the 0.1% BPB solution‚ six diluted solutions‚ ranging from 1:50 to 1:10000‚ were prepared. Each solution was then run in the UV spectrophotometer to measure the absorbance at 590nm. DI water was used as a blank and samples were measured starting from the least concentrated one. The graph of concentration versus absorbance was plotted from the obtained data. In the second part‚ each LB plate was inoculated with DH5α and DH5α pUC18‚ respectively. Two LB/Amp agar plates were treated in the similar
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Determining Red 40 Concentrations Using Absorption with Beer’s Law Introduction I like color and enjoyed learning about wavelengths and the spectrum of light‚ so I considered incorporating something related to that into my Internal Assessment. We also had just used concentrations in our Group 4 Project‚ so when I found an experiment that dealt with both of these I thought it was a great idea. This experiment is not completely original; the basic concept has been used multiple times. It uses Beer’s
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pg.2 WATER FLOW ACROSS CELL MEMBRANES The movement of water across semi-permeable membranes as it relates to the laws of thermodynamics and energy flow with the diffusion of molecules down a concentration gradient of various solute concentrations‚ observing microscopically viewed outcomes‚ and spectrophotometrically measured results. ABSTRACT This experiment will identify the movement and exchange of water through semipermeable membranes and the recognizable amount
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medium and the concentration of the mixture. The principle of calorimetry is the discipline or act of calculating changes in limits of chemical reactions‚ physical changes‚ and phase transitions for the intention descending the heat or heat transfer connected to those changes. The purpose of the experiment is to determine the concentration of an unknown using Beer’s Law‚ also to determine the concentration of blue dye #1 using visual colorimetry and the concentration of blue dye #1
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Linearity: A linear correlation coefficient factor was obtained between the peak area used and the absorbance Verses concentrations of lamivudine‚ zidovudine and nevirapine. The calibration curves were linear for concentrations between 15-150 µg/ml. The linearity of the calibration curves was validated by the values of the correlation coefficients (r2). The correlation coefficients were 0.999 for lamivudine‚ 0.999 zidovudine and 0.999 for nevirapine. The results of the linearity experiment are listed
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Fresh potatoes extract in phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts
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Professor Shannan Lewis-Blair‚ M.S. Submitted By: Kara Hackett‚ Ryan Ritchie & Angie Wagoner Kara_Hackett@pba.edu‚ Ryan_Ritchie@pba.edu & Angie_Wagoner@pba.edu Date of Submission: September 10th‚ 2013 Title of Assignment: Barriers to Concentration CERTIFICATION OF AUTHORSHIP: I certify that I am the author of this paper. This paper was prepared by me specifically for this course. I have also cited any sources from which I used data‚ ideas‚ or words‚ either quoted directly or paraphrased
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