Kinetics of a Reaction I. List of reagents & products 1. 1.0 M Copper(II) nitrate (Cu(NO3)2‚ 0.10 M Hydrochloric Acid (HCl)‚ 0.010 M Potassium Iodide (KI)‚ 0.040 M Potassium Bromate (KBrO3)‚ 0.0010 M Sodium Thiosulfate (N2S2O3)‚ 2% Starch solution‚ Water (H2O) II. Summary of Procedure. Part 1: Find the Volume of One Drop of Solution 2. Fill pipet with 3ml of distilled water 3. Mass a beaker and record 4. Put 5 drops of water into beaker and record
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PCR: 1 μL of Herculase II Fusion DNA polymerase‚ 10 μL of 5X Herculase II reaction buffer‚ 0.5 μL of a 100mM solution of dNTPs (25 mM each)‚ 2 μL of a 10 μM solution of each primer‚ 100 ng of each upstream and downstream fragments‚ 200 ng of pyrG marker fragment and adjust to 50 μL of double-distilled water. 35. Lysis Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of 1 M Tris-HCl pH 7.0 (121.14
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The Grignard Reaction Abstract Through the use of the Grignard reaction‚ a carbon-carbon bond was formed‚ thereby resulting in the formation of triphenylmethanol from phenyl magnesium bromide and benzophenone. A recrystallization was performed to purify the Grignard product by dissolving the product in methanol. From here‚ a melting point range of 147.0 °C to 150.8 °C was obtained. The purified product yielded an IR spectrum with major peaks of 3471.82 cm-1‚ 3060.90 cm-1‚ 1597.38 cm-1‚ and 1489
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Properties of Chemical Reactions Ms. Whitty‚ Science 10 Fiona Adams‚ November 1st‚ 2012 Introduction Chemical reactions are a part of our daily lives‚ from rusting metal to making bread to leaves changing colour in the fall. A chemical reaction is the process that occurs when two or more substances combine to produce a chemical change. When a chemical reaction takes place‚ the change is indicated by one or more qualitative properties. The colour or odor could change‚ gas could be produced
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Lab Report: Identifying Physical and Chemical Changes Nelson Science Perspectives 10 section 5.2 Amazing Student September 17‚ 2012 Mrs. Volkmann SNC 2D Purpose: To collect evidence and then use it to identify physical and chemical changes. Hypothesis: I predict the following: Change 1 will be a chemical change Change 2 will be a chemical change Change 3 will be a physical change Change 4 will be a chemical change Change 5 will be a chemical change Change 6 will be a physical
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affecting the rate of reaction‚ PNPP (p-nitrophenyl phosphate) + H20 ? PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase‚ which alters its active site‚ and thus changes its efficiency to catalyze the reaction. We measured the rate of reaction‚ by using a chromogenic
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Purpose: To investigate the difference between physical and chemical changes in matter. Materials: Safety glasses 13 small test tubes Test tube rack Test tube holder 2 100-mL beakers Funnel Watch glass Stirring rod Evaporating dish Magnet Magnifying glass Crucible tongs Spatula Balance Meeker burner Ring stand Iron ring Wire gauze Forceps Sulfur Iron filings Sodium Bicarbonate Sodium Chloride Sugar Sand Magnesium ribbon Hydrochloric Acid Distilled water Filter paper
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which complexes with the sulfide radical to create an H2S2•- species.2 This type of reaction can continue to create polysulfide chains.2 Lastly‚ type III reactions
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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Introduction The purpose of this experiment is to determine the rate equation for the “Iodine Clock Reaction” experiment. The experiment will consider the equations 〖2I〗^-+S_2 O_8→2〖〖SO〗_4〗^(2-)+I_2 and I_2+2〖S_2 O_3〗^(2-)→2I^-+S_4 O_6 in order to determine the rate law of Rate=k[〖〖S_2 O_8〗^(2-)]〗^a 〖[I^-]〗^b by using the experimental data to calculate the values of exponents a and b as well as the rate constant k. Experimental Supplies Needed: 250 mL Erlenmeyer flask‚ 100 mL beaker‚ graduated
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