In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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Every cell‚ whether it belongs to a plant‚ bacteria or a human‚ requires energy from varying food sources to survive. However‚ when cells break down food into its sugar components‚ i.e glucose‚ they cannot use the components directly because glucose holds a lot of energy it would be inefficient to use for reactions that don’t need a lot of energy. Imagine you want to buy pencils for $5 from a store that won’t give change‚ it is more economical to use a $5 or $10 bill than a $100‚ that way there is
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PRE-LAB The purpose of the lab is to determine the moles of hydrogen gas and then molar mass of the metal. First‚ the metal assigned to each group must be weighed out in order to ensure the hydrogen gas produced will not exceed the capacity for the gas measuring tube. Then‚ copper will be wrapped around the metal leaving 6-8cm at the end for a handle. The end wire will be bent into a hook to keep the sample at the bottom of the tube and increase the oxidation rate. A ring stand will need to be
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Lab #28 Conservation of Mass Ashleigh Bublinec Serena Contreras
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mL of unknown #12. The flask was then covered and had a hole poked through the foil. Next‚ it was placed into boiling water. After the liquid was fully evaporated it was taken out‚ wiped dry and‚ allowed to cool. Then the mass was measured. Then the lab station was cleaned and the waste properly disposed of.
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The objective of this experiment was the synthesis of Isopentyl Acetate using an esterification reaction between acetic acid and Isopentyl Alcohol‚ using a strong acid as a catalyst. The product was washed‚ and distilled. This approach is called Fisher esterification‚ whereby esters are produced by the esterification of a Carboxylic acid where it is heated with an alcohol in the presence of a strong acid which acts as a catalyst. The ester produced had a banana flavor. The extraction of the
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Synthesis and Purification of Acetylsalicylic Acid (ASA or Aspirin) Background Salicylic acid is a phenol as well as a carboxylic acid. It can therefore undergo two different types of esterification reactions‚ creating an ester either with the hydroxyl or with the acid. In the presence of acetic anhydride‚ acetylsalicylic acid (aspirin or ASA) is formed. Correspondingly‚ an excess of methanol will form methyl salicylate‚ which is also an analgesic. In this experiment‚ we shall use
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5.4. Synthesis of Poly L-Lactide (PLA) PLA was synthesized by the ring opening polymerization of L-Lactide in presence of stannous octoate. L-Lactide (1.44 g (0.01 mol)) was dissolved in 20 mL of toluene; 1.5 mg of stannous octoate was added to this solution. The
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First‚ the synthesis of the coordination compound‚ KaFeb(ox)cdH2O‚ needed to be carried out. This was achieved by weighing out 8.29 grams of K2C2O4 with a Dial-a-gram balance. Once measured‚ the 8.29 grams K2C2O4 were placed in a 125 mL Erlenmeyer flask. 25 mL of deionized water were then added to the 125 mL flask and stirred with the K2C2O4 until complete dissolution was achieved. Next‚ 4.11 grams of FeCl3 were weighed out using the balance previously mentioned. Once the 4.11 grams were measured
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IB1 Chemistry Practical #8 ANALYSIS OF ASPIRIN TABLETS For a long time the bark of the willow tree (salix alba) was used as a traditional medicine to relieve the fever symptoms of malaria. In the 1860’s chemists showed that the active ingredient in willow bark is salicylic acid (2-hydroxybenzoic acid) and by 1870 salicylic acid was in wide use as a pain killer (analgesic) and fever depressant (antipyretic). However‚ because it is a relatively strong acid‚ salicylic acid has the undesirable side effect
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