pooled and concentrated in vacuum at 40°C using a rotary evaporator. The crude extract was then diluted in minimum amount of chloroform (2ml) and citrinin was estimated by thin layer chromatography (TLC) according to Razak et al.[16] Subsets of citrinin obtained were estimated by High performance Liquid Chromatography (HPLC). The control contained PDB broth and 10 μl of fungal suspension. Briefly‚ different volumes (1 to 5 µl) of sample extracts were applied to precoated
Premium Chemistry Water Oxygen
antioxidant. Lycopene is found in various fruits and vegetables. The purpose of this experiment is the isolation of Lycopene from tomato paste. This is done using liquid/solid extraction and chromatography. Once the Lycopene is isolated‚ IR spectroscopy will determine its percentage actually obtained by chromatography. Procedure: A massed sample of 1.012g of tomato paste was placed in a 125mL Erlenmeyer flask. To the flask‚ 5mL of 50:50 hexane-acetone was added into the flask. After the 50:50
Premium Lycopene Laboratory glassware Photosynthesis
strains of Halobacteria were used in this experiment to examine the phenotypic differences against each strain’s genotypes. The mutant strain (KBT-1) did not possess gas vesicles‚ which decreased its ability to float to the surface. The lack of gas vesicles in the mutant strain made the colonies a red color. The wild type strain (NRC-1) had gas vesicles and appeared a pink pigment color. Growth on the agar allowed one to examine the specific colonies. Inoculation and growth in the liquid culture were done
Premium Bacteria Microbiology DNA
Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
Premium Bacteria Gram staining Microbiology
will release when the candle is extinguished burn. Procedure: 1. Light the candle 2. Let it burns for about a minute 3. Light a match 4. Blow the candle out 5. Place the lit match about one inch above the wick in the path of the gas. Try again if there is nothing happened. If it does‚ see if the same thing happens if the lit math is held higher
Premium Hypothesis Hydrocarbon Scientific method
ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
Premium Oxygen Hydrogen peroxide Enzyme
Formal Lab Report | Chemistry | | Student name: Sara Hattar Grade: 10 ”C” | | Teacher: Christine Ziadat | | | Title: Investigating the effects of changing the concentration of an acid on the rate of reaction between HCl and Magnesium. Aim: In this experiment we are going to see the effects of using three different concentrations of HCl when added to Magnesium metal on the rate of reaction. Hypothesis: If the concentration of the acid increased in reaction between HCl (the acid)
Premium Chemical reaction Chlorine Chemistry
et al Abstract: The purpose of this lab was to determine how light affected photosynthesis‚ specifically the production of O2 bubbles. It was predicted that when the light was more intense the O2 bubble production will be high. Conversely‚ when the light was less intense the O2 bubble production will be lower. Basically the plant that is closer to the light will produce more bubbles than the plant that is placed farther away from the light source. In this lab the independent variable is the light
Premium Photosynthesis Plant Light
and natural gas sector comprises of exploration and production of crude oil‚ natural gas‚ refining and marketing of the petroleum products‚ which are classified as upstream‚ downstream and midstream. Industrial Research on India - Oil & Gas Sector July 2012‚ BIM Trichy Arjun Venugopal‚ Divakaran‚ Revathy Prasad‚ Sreejesh N‚ Vincent Ministry of Petroleum & Natural Gas Government of India Domestic Production Natural Gas (52.22 bcm) Petroleum (37.7 MMT) Imports Natural Gas (13 bcm) Petroleum
Premium Petroleum
ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the
Premium Thin layer chromatography Red Green