Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
Premium Ultraviolet DNA Plasmid
Gilgamesh’s Transformation The Epic of Gilgamesh is an enlightening story that is filled with knowledge and wisdom that can teach everyone . Gilgamesh shows a great change from the beginning of the epic to the end‚ which can teach us all a lesson about life and death‚ and more importantly about our lives and how we should or shouldn’t live our lives. At the beginning of the story we see Gilgamesh as someone who thinks he is better than everyone‚ who treats his people unkindly and who uses people
Free Epic of Gilgamesh
This front sheet must be completed by the learner where appropriate and included with the work submitted for assessment. Unit: 27 – Dealing with Challenging behaviour Course: BTEC National Extended Diploma in Health and Social Care (level 3) Learner Name (Please enter your name): Jade Monk Assessor Name: Jayne Boyns Internal Verifier: Lead IV Sampled? (Y/N) Pre-Issue IV Date: Assignment Title: 27b Assignment Ref (if used): Issue Date: w/c End Date: Actual Hand-in Date: Unit/ AC Ref Assessment
Premium Self-esteem Mental disorder Psychology
Good Citizenship Values A good citizen is one who properly fulfills his or her role as a citizen. There are many opinions as to what constitutes a good citizen. Theodore said‚ "The first requisite of a good citizen in this Republic of ours is that he shall be able and willing to pull his weight." Education is sometimes viewed as a prerequisite to good citizenship‚ in that it helps citizens make good decisions and deal with demagogues who would delude them. Roger Solder writes that in a democracy
Premium Citizenship Government
Citric acid is used mainly for home use‚ for getting rid of lime scale (kettles‚ baths‚ toilets)‚ in hard water areas. Citric acid is a weak acid found in fruits such as lemons and oranges. Like other acids‚ citric acid can help you remove stains and rust deposits from toilet bowls without causing any damage to the porcelain surface. Citric acid contains just enough acidic concentration to get the job done without etching or damaging household surfaces in any way. It doesn’t harm the environment
Premium Acid dissociation constant Acid Acetic acid
waist with rounded hips and bosom‚ which women attained through the use of corsets and structured undergarments. The dresses were floor length and often designed with long trains and high necklines to ensure maximum coverage. The fashions did not change significantly according to season‚ often causing fainting spells when the summer heat became overwhelming. Towards the end of the century‚ doctors began to warn against the use of corsets‚ which they discovered had the potential to mutilate the bodies
Premium World War II Roaring Twenties Haute couture
Japan’s Lost Decade : An Introduction Japan’s economy has endured the first lost decade during the 1990s. Looking at the current situation‚ experts are pointing out that Japan is now experiencing their second lost decade due to similar characteristics of the first which is stagnant‚ non-improving condition of their economy. Did Japan learn anything from the previous lost decade‚ or did the steps taken to avoid another lost decade were too weak? What was the problem? We will try to look into the
Premium Inflation Recession Monetary policy
The Phenomenon that is Genetic Transformation Matt Kimmel November 17‚ 2011 Bio 121-012 Introduction Genetic transformation is when the genetic makeup of an organism is altered by it receiving external genetic material (Barnhart and Hopper‚ 2011). Bacterial transformation was first seen during an experiment by Fredric Griffith in 1928. In the experiment there were two strains of bacteria‚ a virulent strain‚ and non-virulent strain. Virulent simply means disease causing‚ and therefore non-virulent
Premium Gene DNA Bacteria
Energy Transformation Conduction‚ convection‚ and radiation are things that exist in our life. Thanks to that stuff we have things like electricity and things to cook too. I am going to explain things about energy transformation. Heat transfer is the way heat moves through matter to change the temperature of other objects. There are three types of heat transfers‚ Conduction‚ Convection‚ and Radiation. The first kind of heat transfer‚ conduction‚ is heat transferring through direct contact of materials
Premium Heat Energy Temperature
Eliza’s Transformation In the play Pygmalion by George Shaw‚ Eliza experiences a type of transformation. Before Eliza first encountered Mr. Higgins‚ she was a dirty‚ improper‚ poor young girl. During her time with both Mr. Higgins and Colonel Pickering‚ Eliza did change. Her change seems so go in somewhat of a cycle‚ however. For the fist few weeks of her stay she questioned everything that Higgins asked her to do. She simply was unable to see how they would help her. Later‚ Eliza begins to understand
Premium A Little Bit 2008 albums