glycogen and cellulose. 10) Write notes on the 3 main types of column chromatography (not HPLC)‚ explaining their mode of action and principal uses. 11) Write notes on TWO of the following chromatographic techniques: Thin layer chromatography‚ gel (size exclusion) chromatography‚ ion exchange
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important to find this out as it can have serious health complications if blood that is un compatible is mixed‚ either during pregnancy or transfusions. This experiment involved using the techniques of PCR to amplify the DNA collected‚ using electrophoresis to separate the DNA and using markers and positive controls of known size to determine the size of fragments produced‚ which in turn can determine the Rhesus status of a sample of DNA. The results of the experiment showed that both DNA samples
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Analysis of A. fischeri chDNA restriction digestion by Sal I enzyme after isolation and purification via agarose gel electrophoresis Bio 219-064 (Techniques in Molecular Biology) Group 5: Tung Nguyen‚ Uyen Tran‚ Amber Beckley‚ Danielle Exler Department of Biology‚ Drexel University‚ Philadelphia‚ Pennsylvania 19104 Submitted October 27‚ 2014 ________________________________________________________________________ Abstract Bioluminescence is one of the benefits that a deep sea organism received
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present in the reaction seen with the intensity of colors cab be read by ELISA reader. [pic] Types of ELISA ■ Direct ELISA ■ Indirect ELISA ■ Sandwich ELISA ■ Competitive ELISA ■ Multiplex ELISA [pic] AGAR GEL
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The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged. To which electrode pole of the electrophoresis field would you expect DNA to migrate Explain. 7. What color represents the negative pole 8. After DNA samples are loaded into the sample wells‚ they are forced to move through the gel matrix. What size fragments (large vs. small) would you expect to move toward the opposite end of the gel most quickly
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Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make 1x TAE 20 L‚ add 400 ml 50X buffer into 19.6 L ddH2O. 4. SDS-PAGE Gel Solutions Vol (L) Tris (g) HCl (ml) 10% SDS (ml) 4x Lower gel buffer 1.5 M Tris-Cl‚ pH 8.8‚ 0.4% SDS 2 363.3 50-60 80 ml 4x Upper gel buffer 0.5 M Tris-Cl‚ pH 6.8‚ 0.4% SDS 2 121.1 70-80 80 ml 4.1 10% SDS 1L: 100g
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DNA profiling is a method of identifying an individual by unique characteristics of their DNA. A specific DNA pattern‚ called a profile‚ is obtained from an individual or a sample of tissue. This allows the comparison of the base sequence of two or more DNA samples to determine whether they are related. DNA profiling has many uses‚ in prevention of economic fraud‚ dietetic work‚ and classifying species‚ identifying bodies‚ forensic science‚ screening for disease‚ and investigating paternity.
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A 0.8% agarose gel was used as the medium for the DNA solutions because it has a better separation of large DNA fragments due to the larger pores throughout the gel. The electrodes on each end of the apparatus allowed the fragments to migrate across the gel. The opposite end of the DNA-containing wells has a positively charged electrode that attracts the DNA‚ which is negatively charged due to its phosphate backbone. Electrophoresis confirmed whether or not the DNA samples
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related laboratory technique by which these segments can be illustrated. In RFLP analysis‚ the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel electrophoresis. Although now largely obsolete due to the rise of inexpensive DNA sequencing technologies‚ RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. In addition to genetic fingerprinting‚ RFLP was
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Abstract: Ultraviolet (UV) radiation and its detrimental effects on plant life have been widely researched. This research studies the effects of UV radiation on sex-determination in Ceratopteris richardii. Sex determination in Ceratopteris richardii is determined by antheridiogen‚ a pheromone that promotes maleness. In this experiment‚ UV radiation was used on the wild type and mutants Her1‚ which are insensitive to the male-inducing pheromone (does not form male gametophytes). Ceratopteris richardii
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