Biology‚ 8e (Campbell) Chapter 20 Biotechnology Multiple-Choice Questions Figure 20.1 1) Which enzyme was used to produce the molecule in Figure 20.1? A) ligase B) transcriptase C) a restriction enzyme D) RNA polymerase E) DNA polymerase Answer: C Topic: Concept 20.1 Skill: Application/Analysis 2) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has
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SDS-PAGE is a standard technique for determining the abundance and molecular weight of a protein using an anionic detergent (sodium dodecyl sulfate or SDS) which gives the molecules a net negative charge. The charged molecules are pipetted into a gel and then
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Abstract: Cajanus cajan (L.) is one of the Pigeon pea cultivated in tropical and subtropical areas. It contains many fractions characterized by their bioactivity and efficacy to enhance biological activities. The aim of the present study was to fingerprinting the Cajanus cajan plant ‚ then extract the flavonoids fraction (FFCC) with methanol extract‚ and assess the antimutagenic efficacy of the fraction extract to reduce genotoxicity induced by cyclophosphamide (CP) using chromosome aberrations‚
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Lab Report #6 Electrophoresis: Using Electricity to Separate Molecules Answer the following questions about the results of the virtual lab activity. Record your answers in the boxes. Activity 1 – Calibration Record your data from Activity 1 in the boxes below. Enter the data (number of bands‚ description of the band patterns) you collected for the protein ladder in the appropriate columns for the gel concentrations (8%‚ 10%‚ 12%) Gel 8% 10% 12% Number of Bands 4 5 6 Description of Band
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3: The results of Experiment 3; Protein Precipitation Precipitant Observation TCA White fluffy precipitate in large mass‚ the fluffy mass goes from the bottom to the top of the tube. The solution was thin and loose. Ammonium sulphate White gel-like precipitate‚ the solution was thick. HCl White precipitate accumulated at the top and also the bottom of the solution. The solution was thin. NaOH No precipitate seen‚ the solution was cream in color. Copper sulphate Light blue coagulated
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Insertion and amplification of EGFP protein into pET41a(+) Plasmid Introduction The overall purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli)‚ a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way‚ from cloning and using recombi- nant research‚ to achieve recombinant DNA
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Population Genetics of the Alu Insertion on the PV92 region of Chromosome 16 Abstract: PCR is a laboratory method used to amplify a small‚ specifically targeted‚ amount of DNA. It has three steps‚ the denaturing of the template DNA‚ the annealing of the primers to the DNA templates and the extension of the new DNA by Taq DNA polymerase. The Alu insert on the PV92 region of chromosome 16 is targeted and its frequency is measured according to the Hardy-Weinberg equilibrium in the Vanier HTK population
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polymorphisms (RFLPs) at specific binding sites. Gel electrophoresis is used to move the RLPF fragments from the negative to positive pole using an electrical charge. The movement of the RFLP fragments distance depends on their size and shape; therefore‚ the heavier ones do not move as far as the denser ones. DNA samples were retrieved from the Biker man and Maroon Flash’s shirts to compare Reveille’s DNA and her fur using the gel electrophoresis using a ladder sample of DNA as the controlling variable
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Polymerase Chain Reaction (PCR) – A technique for amplifying DNA in vitro by incubating with special primers‚ DNA polymerase molecules‚ and nucleotides. 12. Gel Electrophoresis – The separation of nucleic acids or proteins‚ on the basis of their size and electrical charge‚ by measuring their rate of movement through an electrical field in a gel. 13. Restriction fragment length polymorphisms (RFLPs) - Differences in DNA sequence on homologous chromosomes
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face that will suggest someone to remember/recognize the features of this particular person. Two tests/types of analysis that can be completed using the bone samples are gel electrophoresis and examining the skull. From running a gel we can learn if the DNA of this person matches the DNA of the suspected missing persons since on the gel there are several lanes that will include a DNA marker and the suspect’s DNA. What we can learn from examining the skull is the age of the person and possibly the ethnicity
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