Introduction: The purpose of this experiment is to see the anti-cow antibody bind to cow serum only‚ and we expect to see the anti-cow antibody bind to the spot that had the cow serum. The system we use in this experiment is the serum from Cow‚ Horse‚ Goat‚ Sheep‚ and Donkey‚ Chicken. In order to able to detect and analyze proteins based on their ability to bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common
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Introduction An essential oil is a concentrated hydrophobic liquid containing volatile aroma compounds from plants. Essential oils are also known as volatile oils‚ ethereal oils or aetherolea‚ or simply as the "oil of" the plant from which they were extracted‚ such as oil of clove. Volatile oils are the odorous and volatile products of various plant and animal species. As they have a tendency to undergo evaporation on being exposed to the air even at an ambient temperature‚ they are invariably termed
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Active transport: requires that a cell expend energy to move molecules across a membrane against the solute’s concentration gradient (the side where it is more concentrated) Feedback inhibition: Metabolic reaction is blocked by its products. A product acts as an inhibitor of one of the enzymes in the pathway to regulate metabolism. Centriole: a structure in an animal cell composed of microtubule triplets arranged in a 9+0 pattern. An animal usually has a pair of centrioles within each of its centrosomes
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Iron is a most significant constituent of the cell redox schemes such as hemeproteins including cytochromes‚ catalase‚ peroxidase and leghemoglobin and iron sulfur proteins like acontiase‚ ferredoxin‚ and superoxide disumutase (SOD) (Marschner‚ 1986). The appearance of iron toxicity symptoms in leaf tissues arises only under flooded state‚ which involves the microbial reduction
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Patients afflicted with diabetes are at risk for a variety of pathologies resulting in many complications including foot ulceration and amputation. The multi-factorial etiology of diabetic foot ulcers is evidenced by the numerous pathophysiologic pathways that can potentially lead to this disorder. A multicenter study attributed 63 percent of diabetic foot ulcers to the critical triad of peripheral sensory neuropathy‚ deformity‚ and trauma (Reiber‚ et al.‚ 1999). The following discusses the pathophysiology
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following components along with inorganic constituents and other unknown microbial metabolites (Table.d). Table.d: Components of Kombucha and its beneficial effects Components Beneficial effects Various Enzymes (Tietze‚1996) Produce enzymes such as glutathione; a powerful
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Conditions That Can Affect Blood Results Using Luminol The purpose of this science experiment was to discover under what conditions can blood give a false result using the presumptive test called luminol. In order to better grasp the experiment‚ research was conducted on several subtopics: the basic concept of luminol‚ the basic concept of artificial blood which is being used for this experiment instead of legitimate blood‚ and substances interferences that could tamper with crime scene results
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CHAPTER II RELATED LITERATURE Blumea balsamifera(sambong) A Phytochemical and Pharmacological Review Sambong (Blumea balsamifera) is a native flowering or weed that is endemic in the Philippines and other tropical countries. It is a popular herb especially for its healing properties including antidiarrhetic‚ antigastralgic‚ expectorant‚ stomachic‚ and antispasmodic‚ among others. Aside from these‚ sambong is also popular for being emmenagogues‚ or for stimulating menstruation or the blood flow
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Intracellular free calcium levels: Intracellular free calcium levels were estimated according to the method of Luo and Shi (Luo and Shi‚ 2005). Briefly‚ hippocampi were isolated from mice brains and subjected to hippocampal cells isolation as described later. The cells obtained were incubated with Fura-2 AM at 37°C with gentle shaking. The fura-2 loaded suspension was centrifuged for 10 minutes‚ pellet was washed once with Ca2+-free buffer and was centrifuged again. Aliquots of the washed suspension
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The results of the experiments carriedout for the degradation of fabric dyes from aqueous solution using Chara and Chara beads are discussed below.Various analytical techniques like UV-Vis‚ FTIR spectroscopy were used to confirm the decolorization as UV-Vis spectroscopy; FT-IR spectroscopy and toxicity bioassay were truly used [14]. Also‚ Fourier transform infrared spectroscopy (FT-IR) is widely used to confirm the product obtained after decolorization of dye degradation experiments [15]
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