Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
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Chapter I THE PROBLEM AND REVIEW OF RELATED LITERATURE AND STUDIES Introduction Everyone is naturally pre-determined to have an offspring. This would just exist in a relationship of a man and woman. It is not always true in a relationship if there is a new life that will be formed. Several factors may contribute to the hindrances in the formation of new life especially to man because male (sperm) gamete is the one responsible to initiate fertilization‚ the union of male (sperm) and female (ovum)
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Classification of Epithelia Lines Examples Simple squamous Surfaces involved in passive transport Alveoli Endothelium Loops of Henle Simple cuboidal epithelia Surfaces involved in secretion and absorption Some glands Ducts Collecting tubule of kidney Simple columnar epithelia Surfaces involved in high rates of secretion and absorption Protection Stomach and small intestine where it has microvilli Collecting tubule of kidney Fallopian tubes where it is ciliated Pseudostratified Usually ciliated
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dissolved‚ the microcentrifuge‚ the thermal cycler‚ the primer solution‚ the automatic cycler‚ the agarose gel‚ whether or not the electrophoresis apparatus was set up and used properly‚ the loading of the DNA samples in the wells of the gel bed‚ proper staining of the DNA‚ and finally the operator error. Our hypothesis is 50 % of the subjects DNA samples contain the genotype. Our null hypothesis is that the genotype is there by chance and there is no genotype in these samples. Experiment/ Methods
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DMLS/FT/1B/02 Admin number: P1138687 Summary This experiment aims to observe mitosis and meiosis on cell specimens. It involves the modelling of the stages of meiosis and also the staining procedures to identify mitosis in onion root tip. There are two parts to the procedure of the experiment whereby the first is staining and observing mitosis in onion root tips and the second being observing and comparing mitosis between whitefish blastula and onion root tip. The results are that the five stages of
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They also used flow cytometry to help them to identify and sort through the cells and their DNA by staining them with a fluorescent dye and detecting the fluorescence through a illumination microscope. This helped them to calculate the number of replication forks per cell‚ along with the cells age and size at initiation. The researcher did an excellent job describing each media and staining technique that they used during their experiments. For example‚ they explained exactly how much nutrient
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Bacterial Biology █ BRIAN D. HOYLE An understanding of the fundamentals of bacterial biology is critical to bacteriologists and other forensic investigators attempting to identify potential biogenic pathogens that may be exploited as agents in biological warfare or by bioterrorists. Fundamentals of Bacterial Biology Bacteria are one-celled prokaryotic organisms that lack a true nucleus (i.e.‚ a nucleus defined by a membrane). Bacteria maintain their genetic material‚ deoxyribonucleic
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Study guide for Microbiology Chapter 2 Name________________ 1. List and describe the sequence steps routinely used to identify bacteria. These are referred to as the “five I’s” in your text. 2. Define / describe each of the following as they apply to microbiology: a. Culture f. pure culture b. Inoculum g. contaminated culture c. Inoculation h. mixed culture d. Colony 3. Microbiologists employee a number of approached to acquiring a pure culture
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TESTING AND SPECIFICATION OF DRYMIX MORTAR IN SINGAPORE ABSTRACT Singapore is a small island country‚ but has won renown as uncorrupted government and high quality products. Independent testing and inspection played a very important role for this achievement. The Housing and Development Board (HDB) is the sole public authority in Singapore and was set up as a statutory on 1 February 1960. Within 45 years‚ it built more than 700‚000 flats housing approximately 86% of the population. Every
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Microbiology Kit (MBK) – Lab Report Name: Section: Observing Bacteria and Blood Questions: A. List the following parts of the microscope and describe the function of each A. Eyepiece lens B. Tube C. Revolving nosepiece/turret D. Objective lenses E. Stage F. Iris Diaphragm G. Illuminator H. Coarse Focus I. Fine Focus J. Arm K. Stage Clips B. Define the following microscopy terms: a. Focus: A means of moving the specimen closer or further away from the
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