of bacterium that allows it to survive poor environmental conditions are called endospores. Their tough outer covering is made of keratin and is also the reason they are resistant to heat and chemicals. The strength of keratin also makes it resist staining. Due to this drastic measures like steaming the bacterial emulsion is used to force primary stains such as malachite green into endospores in the Schrieffer Fulton method. This stain can be left on the slide for 15 minutes or more to stain the spore
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bath to flatten the sections. The sections were picked up onto the microscope slide‚ drained it in vertical position for several minutes. The slides were then placed on a slide holder in the oven at 37 °C overnight. Step 5: Staining The slides were placed in a staining rack and were dipped into xylene for 5 minutes and repeated once in new xylene for another 5 minutes. Then‚ the slides were dipped into
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8.1 MICROWAVING Principle: Microwaving is a method used for heating of tissue sections and cellular components with the use of a microwave which provides heat by electromagnetic waves that has a high frequency‚ with a wavelength if one millimetre to one meter and an intermediate between infrared and shortwave radio wavelengths (Miffin‚ 2002). This allows fast fixation. Technique: Electromagnetic waves are commonly used for carrying out energy and are said to be powerful for non-invasive processing
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makes sense of the fundamental concepts of unity and diversity among living things ○ Basic principle is that members of higher-levels groups share fewer characteristics than those in lower-level groups * Escherichia coli - rod shape and have a Gram-negative cell wall * Even members of the same species display variations in size‚ shape‚ and other characteristics Binomial Nomenclature • Carolus Linnaeus - credited with founding the science of taxonomy ○ Originated binomial nomenclature
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Smoking - effects on your body Summary Nicotine is the addictive drug in tobacco smoke that causes smokers to continue to smoke. Addicted smokers need enough nicotine over a day to �feel normal� � to satisfy cravings or control their mood. How much nicotine a smoker needs determines how much smoke they are likely to inhale‚ no matter what type of cigarette they smoke. Along with nicotine‚ smokers also inhale about 7‚000 other chemicals in cigarette smoke. Many of these chemicals come from burning
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Title: Cell Structure І Objectives: 1. Prepare specimens for staining. 2. Identify unique and standard structures of the microscopic world. 3. Identify and use different stains for the different types of organelles. Results: a) Epidermal cells of plants: Upper surface or Rheo discolours leaf Low power: 40X Upper surface or Rheo discolours leaf Low power: 40X Upper surface or Rheo discolours leaf High power: 100X Upper surface or Rheo discolours
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Explain the systems within your dental practice for internal and external referrals for advice‚ support or treatment. The most common referral in our practice is for a therapist for scale and polish‚ filling‚ whitening teeth‚ radiographs or for extraction of deciduous teeth. Our therapist doesn’t have direct access and patients have to be referred to her. To refer a patient to the therapist‚ appointment need to be booked and therapy prescription placed on the top of the notes in patient file with
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scrumptious dish. When these pale‚ yellowish noodles are done cooking‚ the only thing left to do is pour that delicious sauce on them. Children have a great time trying to slurp and gobble up the slippery noodles‚ while the parents try to keep the ever staining red sauce off their faces. Negative Description: Cooking spaghetti is a long and tiring process. One might think all you do is boil water‚ put the noodles in‚ and slap some sauce on top. But to get the best tasting sauce‚ it has to be homemade
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stored at room temperature (approximately 25°C). Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety precautions and under the supervision of a person familiar with such precautions. Use of this kit by unsupervised or improperly supervised individuals could
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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