This experiment consisted of two parts. First‚ the absorption of each dye was evaluated by diluting (1 mg/mL standard) 2.0 mL of dye to 100 mL. The absorbance of each dye standard was measured and documented. The absorbance of the Gatorade was then measured by diluting the solution and pipetting 5.0 mL into a 25.0 mL volumetric flask. The absorption spectrum of the chosen drink was measured. Next‚ the purple Gatorade extraction procedure was carried out‚ beginning with pipetting 1 mL of 70% isopropanol
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The purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in
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The lipase gene sequence from Alcaligenes sp. JG3 was translated into amino acid sequence using two methods‚ manual translation and ExPASy online software. By manual method‚ amino acid sequence was obtained via codon translation one by one as shown in Figure IV.7. The sequence reference used is the lipase from A. faecalis MOR02 due to having high similarity with the lipase sequence from strain JG3 during CLUSTAL alignment and the alignment of the deduced amino acid with the referred sequence was
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In this section we will analyze which steps were the most effective ones in recovering LDH (percent yield) and in purifying LDH (fold purification). As we can see looking at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion
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1. What is the more formal chemical name for aspirin? Acetylsalicylic acid 2. Using your data from Experiment 2‚ how can you be sure you synthesized aspirin? By using the colors as a guide‚ we can compare the Acetylsalicylic acid + 8 mL FeCl3 to the Aspirin Crystals from Experiment 1 + 8 mL FeCl3 in order to see if it synthesized. 3. Would you say that your synthesized aspirin is relatively pure? How can you tell? One way that the purity of aspirin can be judged is by it’s color as pure
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2.3. Method Development 2.3.1. Preparation of Stock Solution 100 mg of the synthesized compound was made up to 100 ml into a 100ml standard flask with ethanol to give a solution of 1000 μg/ml. 2.3.2. Determination of λ max 1 ml of stock solution was pipetted out was made up to 10 ml into a 10ml standard flask with ethanol to obtain strength 100 μg/ml and scanned at 200-400nm using a UV spectrophotome-ter (Deepak V Bageshwar et al.‚ 2010). 2.3.3. Preparation of Standard Calibration Curve Aliquots
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Organic Lab 2 February 14‚ 2009 Experiment 4: Borohydride Reduction of 2-Methylcyclohexanone Introduction: When learning about reduction reactions‚ we know that the addition of nucleophilic hydride anion (H-) to the carbonyl group will in turn result in the reduction of aldehydes to primary alcohols. Borohydride‚ and Lithuim aluminum hydride are commonly used as reducing agents. We can note that both of these reagents have a (H-) anion‚ hence it will be a powerful base and also take the
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The following paragraph describes the procedures and steps taken on day one of the experiment‚ which focused entirely on the synthesis of the isomer based on Protocol B. Certain chemical agents used on day one have both health and safety risks‚ specific details regarding these risks will be explained in more detail in the following procedures‚ but if unfamiliar with any of these
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Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form pairs
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The objective of this experiment was the synthesis of Isopentyl Acetate using an esterification reaction between acetic acid and Isopentyl Alcohol‚ using a strong acid as a catalyst. The product was washed‚ and distilled. This approach is called Fisher esterification‚ whereby esters are produced by the esterification of a Carboxylic acid where it is heated with an alcohol in the presence of a strong acid which acts as a catalyst. The ester produced had a banana flavor. The extraction of the
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