DISCUSSION In this experiment‚ we will extract plasmid DNA that carrying pKan and pAmp from E. coli that have been cultured overnight in LB media containing antibiotic kanamycin and ampicillin respectively. So‚ the DNA that will be introduced later will make the bacteria resistance to antibiotic kanamycin or ampicillin. A plasmid is a small‚ circular‚ double stranded DNA molecules and cloning vector that are widely used for recombinant DNA technology. It can be physically separated from chromosomal
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Investigating the Kinetics of the reaction between Iodide ions and Peroxodisulphate (VI) ions By the use of an Iodine clock reaction I hope to obtain the length of time taken for Iodine ions (in potassium iodide) to react fully with Peroxodisulphate ions (in potassium Peroxodisulphate). I will do three sets of experiments changing first the concentration of iodide ions‚ then the concentration of Peroxodisulphate ions and finally the temperature of the solution in which the reaction is taking place
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2013 “Reaction Classes and Pericyclic Reactions” ● 4 Lectures ● Recommended Texts: (i) J. March “Advanced Organic Chemistry”‚ p 839‚ `---Edn.‚ Oxford (W); Warren (2) Ch 34‚ 35. Syllabus 1) General principles (a) Reaction classes (i) Heterolytic (ii) Homolytic (iii) Pericyclic (b) Pericyclic Reaction Types 2) Electrocyclic Reactions (a) Definition and examples (b) Stereochemical aspects and the Woodward and Hoffmann rules 3) Cycloaddition Reactions (a) Definition
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A hypo-kinetic disease is a disease that is associated with a lack of physical activity or lack of exercising. Obesity is an example of a hypo-kinetic disease‚ which makes sense because it is a condition of an accumulation of excessive body fat as a result of not creating a caloric deficit as well as not being physically active. Obesity affects the body by changing the normal body composition. It affects cardio-respiratory endurance‚ flexibility‚ muscle endurance and how much power and strength one
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r Virtual Lab: Enzyme Controlled Reactions Instructions 1. Go to the following web-link in order to open the Virtual Lab: http://www.mhhe.com/biosci/genbio/virtual_labs_2K8/labs/BL_02/index.html 2. Open the Virtual Lab: Enzyme Controlled Reactions 3. The virtual lab simulation will be on the right side of the screen‚ and the “Question” column will be on the left side of the screen. 4. Click the monitor in the lab simulation to watch a video about enzyme action. 5. Click the “Information”
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Factors Affecting Reaction Rate Bob Jones Josh October 2‚ 2014 Period 3 Introduction: In reference to the collision theory‚ molecules act as small spheres that collide and bounce off each other‚ transferring energy among themselves when the collide. In order for a reaction to occur‚ there must be collisions between molecules. Through experimentation‚ factors are discovered that influence the reaction rates of chemical reactions include the concentration
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Experiment 16: Kinetics: The Iodine Clock Jane Smith Purpose: The objective of this experiment was for students to apply their knowledge of kinetics and rate laws in order to determine the rate of a chemical reactions‚ activation energy‚ and frequency factors of those reactions. Specifically‚ this experiment was performed using a series of solutions with varying concentrations of KI‚ Na2S2O3‚ and (NH4)2S2O3 . Students recorded time elapsed to observe physical evidence of a reaction taking place
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experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch
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examine a number of different chemical reactions and determine if they are exothermic or endothermic. Apparatus: • Test tubes {Around 10-12 in number} • Test-tube rack • Spatula • Digital thermometer { ± 0.1° C} • Digital balance {± 0.01 g} • Measuring cylinder {± 0.5cm³} • Different chemicals Introduction/Theory: Exothermic reactions are those reactions that release energy in the form of heat. Endothermic reactions need to absorb energy in the form of heat
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