aureus)‚ the serum will coagulate‚ resulting in a clot (sometimes the clot is so pronounced that the liquid will completely solidify). * If negative‚ the plasma remains liquid. The negative result may be S. epidermidis but only a more detailed identification test can confirm this‚ using biochemical tests as in API tests and BBL CRYSTAL methods. MATERIALS Sterile swab‚ oxidase strap‚ microorganisms (Pseudomonas aeruginosa‚ E. coli) METHODS A. Catalase 1. A drop of 3% H2O2 was placed
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Identification of an Unknown Organic Acid Chem 1211K Lab Drawer #15 Wednesday November 13th‚ 2013 Unknown Number: 3334025-CF13 Table of Contents Pg. # Abstract 3 Experimental Report 4-7 Results and Discussion 7-12 Conclusion 13 **pKa Graph 14 Abstract Identifying this organic acid was an extensive task that involved several different experiments. Firstly‚ the melting
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Experiment H: Identification of an Unknown Organic Compound The objective of this lab was straightforward. We were given an unknown compound and we were to perform an IR spectroscopy and as well as NMR spectroscopy. With the IR spectroscopy‚ I was able to name the functional groups I have on my compound and further confirmed my assumptions by looking at the NMR spectroscopy after. The unknown number I was given was number 203. The molecular weight of the compound was 121. From the molecular weight
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The purpose of this lab is to isolate a bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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A Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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Identification of Metallic Ions Peter Jeschofnig‚ Ph.D. Version 42-0160-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor. obseRvations
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Hydrogen Producing Bacteria was incubated in a complete - mix digester with work volume 1.7 L‚ seeded with sludge obtained from the local sewage treatment plant. Each liter of feed medium was composed of the following : 7 g of glucose‚ 1 g NaHCO3 ‚ 500 mg of NH4Cl ‚ 250 mg KH2PO4 ‚ 250 mg K2HPO4 ‚ 320 mg of MgSO4 • 7H2O ‚ 50 mg of FeCl 3 ‚ NiSO4 32 mg ‚ 50 mg CaCl2‚ Na2BO7 7.2 mg H2O ‚ 14.4 mg (NH4) 6MO7O24 H2O ‚ 23 mg of ZnCl2 ‚ 21 mg CoCl2 H2O ‚ 10 mg CuCl2•2H2O and 30 mg of MnCl2•4H2O . The reaction
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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