The first experiment involved agar cubes‚ each with a different surface area‚ to measure how surface area affects the rate of diffusion. In this experiment‚ the constants included 2% agar containing the pH indicator dye phenolphthalein‚ 1% phenolphthalein solution‚ 0.1M NaOH‚ 0.1M HCl‚ squares of hard‚ thin plastic (from disposable plates); unserrated knives; or scalpels from dissection kits‚ metric rulers‚ petri dishes or test tubes to hold the agar cubes. For the independent variable‚
Premium Osmosis Concentration Diffusion
For experiments 1and 2‚ many different tests were conducted in order to investigate the different microbes found on the different types of turkey sampled. To begin‚ our group obtained 5 different forms of turkey that are encountered in everyday life: Inspirations deli turkey from Hannaford supermarkets‚ turkey bacon‚ turkey jerky from Whole Foods‚ Inspirations ground turkey from Hannaford‚ and a raw whole chunk slice of turkey breast form Whole Foods‚ these foods were considered experiment 1. Experiment
Premium Bacteria Water DNA
culture on the EMB-lactose and PEA. Vancomycin wasn’t available to be used. We added our live culture to the EMB-lactose and PEA and added sterile beads to spread the bacterial cells all over the surfaces of the two agars and removed the beads and incubated the two agars. While the agars were incubating‚ we prepared our gel and loaded our respectful samples and ran the gel. After the gel finished running‚ we got a picture of our gel and recorded our observation for later
Premium Bacteria Microbiology Microorganism
Title: Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Title : Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Objective : To screen for thermophilic fungi as producer of fungal cellulase. Introduction One of the most important sources of carbon that is abundantly found on this planet is cellulose. While cellulase is the enzyme to degrade this carbon and it is a key enzyme in the bio refinery process of producing green chemicals
Premium Cellulose Enzyme Fungus
well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow. This experiment allowed us to observe the process of bacterial transformation. I believe that only a small percentage of the cells will transform and the gfp plasmid will be most apparent in the agar plate containing both the plasmid and ampicillin.
Premium Bacteria DNA Escherichia coli
ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
Premium Bacteria Microbiology
Gram-negative bacteria and fungal strains by measuring zone of inhibition. The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5‚ 5.0‚ 7.0‚ 10µg/ml respectively. Ampicillin (antibacterial)‚ Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in
Premium Caffeine Coffee Tea
some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium‚ the bottom of the BHIA dish was labeled with the bacterium number‚ initials‚ and section; then divided into four quadrants. The second step‚ we used the septic technique to transfer a small
Premium Management Learning German language
inoculating loop and dry heat technique‚ isolation streak was performed and nutrient agar plates were to be incubated in a room temperature for the next 48 hours. Nutrient Agar plate was used for isolation streak technique in order to see two types of bacterium growing in a room temperature. After incubating for 48 hours Nutrient agar plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification‚ those
Premium Microbiology Bacteria Staining
procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. This was done by streaking the unknown onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed
Premium Bacteria Enzyme Staphylococcus