The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 4. Using sterile technique place disk in each of the solutions:
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Medical Microbiology II - Laboratory Report 1 Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral
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Microbiology and Process Analysis laboratory 25/10/2013 Group 1 Microbiology laboratory Abstract The lab exercises were divided into three different analysis; microscopy‚ soil microbiology and bacterial growth. The main aim of laboratory work with Escherichia coli and soil sample was to introduce students to bacterial growth in pure culture and soil microbial flora. The experiment of bacterial growth in pure culture using optical density
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The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
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glass tube test and the agar-water gel test. In the glass tube test‚ two cotton balls were soaked in two different substances‚ one in hydrochloric acid (HCl) and the other one in ammonium hydroxide (NH4OH). The cotton balls were inserted into the two ends of the tube at the same time. After some time‚ a white smoke was observed to evolve nearer the cotton ball soaked in HCl. This indicated that NH4OH‚ which is lighter‚ diffused faster than HCl. The next test was the agar-water gel test. It was composed
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Cyra Vose Klebsiella Pneumoniae #34 MCB 2010C Friday 9:00am Introduction: On November 16‚ 2012 at 0900 I was given case #34. Mr. Joe Blough presented with a sudden high fever (103 degrees Fahrenheit or higher) and mentioned he felt dizzy and had the chills. During this visit Mr. Blough seemed to be coughing and producing sputum. This sputum was thick and had some blood present. I had my assistant collect a sample of the sputum so we could run some tests and hopefully correctly diagnose Mr. Blough
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this study. All procedures‚ unless otherwise noted‚ have been followed and performed exactly as stated in the laboratory manual Leboffe & Pierce(1). Using the Quadrant Streak technique the unknown and ubiquity were streaked onto a trypticase soy agar (TSA) plate. This method was performed as stated in the Leboffe & Pierce lab manual(1). The TSA plates were incubated at 30 degrees Celsius for 48 hours. This process was repeated until isolated colonies appeared. At this point cell morphology was
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All strains were provided by Ondokuz Mayıs University‚ the Faculty of Veterinary Medicine and Faculty of Medicine. Agar diffusion test The microbiological assays were performed under aseptic conditions inside a class 2 cabinet (Danlaf VFRS 1806‚ Denmark) previously sterilized. All testing procedures were carried out by a single operator using sterile instruments. Agar diffusion test (ADT) was used for determination of antimicrobial activities of test materials. The microorganisms inoculated
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negative control‚ 15 seconds‚ 30 seconds‚ 45 seconds‚ and 60 seconds). Aseptic technique was critical during each transfer of agars. Plain 50 agar was poured equally to each plate labeled. A cotton end of the applicator stick was wet with the liquid culture of bacteria‚ Serratia marcescens‚ and applied on the surface of agar the same way it was carried out to cover the agar gradient in the first part of the experiment. Plates were prepared for the UV exposure. It was in this step that each plate
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