PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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absence of a capsule. Unknown 45 was positive for capsules. Having determined that U45 was gram-negative‚ there was no need to perform the Schaeffer-Fulton endospore stain‚ which is used detect the presence of endospores in certain gram-positive bacteria. The following metabolic tests were performed on U45 based on instruction given in Microbiology Lab Manual by John P. Harley‚ and by the instructor: 1. Carbohydrate fermentation 2. Sulfide Indole Motility (SIM) test 3.
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Observation of Bacteria on Chicken and Handrail and the Effect of GermX® and Alcohol on Them. Sujit Bhimireddy‚ Daryl Onwuchuruba‚ Naveen Sood‚ and Manasa Tripuraneni Biology 106 Abstract Bacteria are found everywhere on this planet. This experiment is aimed at observing some of the bacteria that surround us in places that we use everyday. We will look at bacteria found on a piece of chicken and bacteria found on a handrail. The effectiveness of antibacterial substances such as GermX® and
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Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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Bibliography: Almeida‚ Raul. Foodborne Pathogens in Milk and the Dairy Farm Environment. N.d. web. 6 September 2012. Goff‚ Douglas. Dairy Microbiology. University of Guelph. N.d. 4 September 2012. Kanade‚ Shrinivas. Pathogenic Bacteria in Milk. Buzzle. 27 August 2011. Web. 5 September 2012. Kelleher‚ Kevin. Bacterial Stains. N.d. web. 24 August 2012. Koo‚ Ingrid. Got Milk Microbes? Medical Review
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performed within a certain time frame the specimen will become contaminated. Part II: Bacterial Anatomy The purpose of this experiment is to become familiar and gain an understanding of how morphology and the arrangement of bacteria relate to staining techniques. Sample 1 In this study a female patient who had a problem that had arisen suddenly after lunch‚ had allergic reaction to something that she ate or drank had sent her to the bathroom with cramps and copious diarrhea. She knew she
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example: “There are many reasons for identifying an unknown bacterium. The reasons range from…[explain 2-3 reasons why you may want to identify an unknown bacterium]. This study was done by applying all of the methods that have been learned in the microbiology laboratory class for the identification of an unknown bacterium.” This section should at least be about 1 -2 paragraphs long. Materials and Methods – this is where the details of the study are listed. Where did the specimen come from and
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symbiotic relationship with its host‚ and for this reason it rarely causes diseases and is usually considered nonpathogenic (Avdic‚ Habes‚ and Avdic 3885). Recently though‚ the microorganism is becoming the common cause of nosocomial infections. In “Microbiology: with diseases by taxonomy”‚ Richard Bauman defines a nosocomial infection as “a disease acquired in a healthcare setting.” These infections are often found with implants and plastic items that have inserted into the body‚ such as catheters‚ pacemakers
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