Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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1. Obtain 1 gram of an impure‚ unknown substance solid. Make sure to stir the mixture before measuring the sample; record the mixture’s code in the data section. 2. Add approx. 25-50 mL of water and several boiling chips to a 125 mL Erlenmeyer flask and heat the water to a gentle boil using a hotplate. 3. On a second hotplate‚ place a 125 mL Erlenmeyer flask containing the one gram of unknown solid along with a boiling chip. 4. Using a ring clamp‚ slowly pour approx. 5-10 mL of boiling water into
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Morales‚ Marinel M. Dr. Annie Cu Gallardo BSE-Biological Sciences III-3 Microbiology (Lecture) Microbiology Written Report REPORT NO. 1 Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some
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Determining pH Using a Red Cabbage Farnoosh Mazarei Food Microbiology April 22‚ 2011 Purpose: To test the pH level of many common household object by use of the juice from a red cabbage. Background: pH is the measure of the acidity or basicity of a solution. The pH scale ranges from 1 to 14 with 1 through 6 being acidic and 8 through 14 being basic; 7 is considered neutral. The use of the red cabbage‚ or red cabbage juice is because red cabbage can be used as an acid/base indicator
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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Crank and Connecting Rod Introduction- The motion of assemblies is determined by the configuration of links and joints. Using the configurations the operation of rotational and sliding joints are examined and observed. This kind of mechanism is very commonplace in machines. Machines are made up of a number of parts and relative motion between the various parts permits the working of the machine. As the crank is rotated the rod starts moving but the velocity is not uniform. It is greater towards
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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Identification and Recreation of Unknown White Compound #643p November 13‚ 2012 INTRODUCTION: The identity of the unknown solid white compound is determined and verified through a series of tests which uncover physical and chemical properties necessary for identification. A new sample of the same compound is then created to further prove the accuracy of the identification. The compound must be identified in order to be used. For example‚ KCl is used in medicine‚ scientific applications‚ and
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Alyse Rose Microbiology Lab Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate
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