with Starch at Specific pH ’s and Temperatures Page 1 Abstract Enzyme ’s are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest‚ any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum temperature and pH for the reaction of Barley
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BRONSTED-LOWRY ACIDS AND BASES 1. The Bronsted-Lowry definition There are many definitions of acids and bases in existence‚ but the most useful one is the Bronsted-Lowry definition: An acid is a substance which can behave as a proton (re presented as a hydrogen ion‚ H+) donor. Any substance which contains hydrogen bonded to a more electronegative element can thus behave as an acid: HCl == H+ + Cl- H2SO4 == H+ + HSO4- A base is a substance which can behave as a proton acceptor. Any
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your response in short-answer format (2–3 detailed sentences each). Use this Phase’s activities and resources to assist you in answering. 7. What is pH? a. pH is a measurement of hydrogen ion concentration. It measures rather the solution is acid‚ base‚ or neutral. 8. What is a base? a. Accepts hydrogen ions from another substance that have a pH>7. It taste bitter and feel slippery. 9. What is an acid? a. Component with a positive or negative charge that break apart in water to form
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4 Titration Curve of an Amino Acid pH Simple amino acid Acidic amino acid Basic amino acid 7 OH- equivalents Objectives: A) To determine the titration curve for an amino acid and B) to use this curve to estimate the pKa values of the ionizable groups of the amino acid and the amino acid’s pI. Introduction: A titration curve of an amino acid is a plot of the pH of a weak acid against the degree of neutralization of the acid by standard (strong) base. Consider the ionization
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LAB 6. ACIDS AND BASES: PH AND BUFFERS PURPOSE: To determine the pH of common acids and bases using a pH meter‚ pH paper‚ and red cabbage indicator. To test the effect of adding an acid or base to a buffer solution. SAFETY CONCERNS: Always wear safety goggles. Wash with soap and water if skin contacts acids or bases. ACIDS: An Acid is a substance that when dissolved in water will produce hydrogen ions‚ H+‚ in the solution. An acid that does not contain carbon is called an inorganic
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pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer Incubation Cond. Boil‚ Inc. 37 deg.C 37 degrees C 37 degrees C 37 degrees C for 60 minutes IKI Test + - - + Benedict’s Test - ++ - - Chart 1 – Salivary Amylase Digestion of Starch (continued) Tube # 5 6 7 Additives DI Water‚ Maltose Amylase‚ Starch Amylase‚ Starch pH 7.0 buffer pH 2.0 buffer pH 9.0 buffer
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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decimal point) Formula used to calculate the average volume of sodium hydroxide used (ml) Average volume of sodium hydroxide used =(Accurate 1+Accurate 2)/2 Part 2: pH titration curves Strong acid-weak base titration Table V: Volume of ammonium hydroxide (ml) pH Volume of ammonium hydroxide (ml) pH 0.00 0.73 20.50 1.73 0.50 1.03 21.00 1.76 1.00 1.04 21.50 1.79 1.50 1.05 22.00 1.83 2.00 1.06 22.50 1.87 2.50 1.07 23.00
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Acids‚ Bases‚ and Buffers Introduction: The pH scale is used to determine how acidic or basic a solution is‚ ranging from 1-14. The most acidic of all acids are at a pH level of 1 and the most basic of all bases are at 14. The neutral pH level is 7‚ which is what drinking water is. The pH level is determined by the amount of H+ ions present in a solution‚ and the more H+ ions there are the more acidic it is‚ and the lack of these ions results in more basic solutions. One distinguishing feature
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Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of the
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