Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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Enzymes are catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site‚ an area in which a material to be acted on by the enzyme‚ called the substrate‚ will fit. The temperature‚pH‚ the concentration of enzyme‚ and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction. The
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Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best results when testing the pH within a solution. The pH‚ which stands for the proportion of hydrogen ions in a solution‚ could be acidic (acidosis)‚ neutral or basic (alkaline). The pH scale goes from numbers 1 through 14. A pH of 7 is neutral; meaning the amount of hydrogen
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This experiment was performed in order to analyze the effects of pH‚ enzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic
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When converted to pepsin‚ the enzyme is used to break down large or undigested protein they has been absorbed by the small intestine. The digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web. 10 Dec. 2012. Research Question: Does changing the pH of pepsin affect the ability to
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The Effects of pH on Slime Mold Dg Nicole Harris BIOL181 – 059 1 December 2011 The theory that slime mold is attracted to acidic solutions was examined by the slime mold‚ Dg. Dg’s preference of pH was examined by using a variety of pH solutions in a petri dish. The data suggests that the growth of fruiting bodies observed in pH 9 was comparable to that of pH 4. INTRODUCTION The 2002 scientific journal‚ “The Costs and Benefits
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effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans Research Question: What is the effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans? Independent Variable: The different pH levels in the water would be the independent variable in this lab. Three different pH levels were tested‚ 4‚ 7 and 10‚ both 4 and 10 were chosen because they are 3 pH levels away from 7 which would be the regular pH level of water. We measured
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Enzymes Lab Report Inroduction In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy‚ which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur‚ yielding products from a given set of reactants. (Unit 7: Enzymes lab) Products
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