EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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Enzyme-Controlled Reaction Procedure: Click the TV/VCR. Then click the Play button on the video controller. Watch an animation about enzyme action. Click More Information to read about enzymes and substrates. To conduct the experiment: Adjust the pH level of the test tube by click the up and down arrows Add substrate to each of the test tubes that already contain an enzyme solution Click and drag a piece of weighing paper with the powdered substrate to a test tube. Click the computer monitor to
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Title: pH and buffer solutions Aim This experiment was carried out to determine the role of buffer solution and the factor which affect the buffer capacity. Besides‚ this experiment was carried out to investigate the solubility of protein casein over a range of pH concentration. This experiment also was carried out to determine the isoelectric point of the casein and the effect of the isoelectric point toward the casein solution. Methods Verification of the Henderson-Hasselbalch equation
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BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site is
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person to their heart in pain‚ and to hold the responsibility for taking their own father to these procedures for his treatment. Cancer nowadays is the most diagnosed disease all around the world. I experienced cancer when my father was diagnosed with Liver Cancer in 2013. It was easy for my family and I to provide him with support to alleviate the emotional pain‚ however‚ we weren’t always able to alleviate the physical pain he went through. Fortunately‚ the medication and treatments were able to help
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BABS1201 Group Enzymes Project Group Protocol Including Equipment and Reagents List Lab Day: Wednesday Lab Time: 10am – 1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: Demonstrator Name: Daniel Winters Names of Group Members: Johnny Nguyen‚ Therese Pham‚ Linda Tang Name of Enzyme You are Investigating: Amylase Brief Background: Amylase is a digestive enzyme‚ produced mainly by the salivary glands and the pancreas‚ to break down
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Enzymes and ATP Enzymes act as protein catalysts in biochemical processes Enzymes bind to a substrate and forms the enzyme substrate complex. Enzymes work by lowering the energy of activation. Activation energy must be supplied for the reaction to begin‚ once supplied‚ the reaction can proceed on its own. Enzymes can speed up events. They are not used by during the reaction because the enzyme stays the same‚ it does not change during the reaction. (Hudon-Miller‚ Enzymes‚ 2013) Enzymes act as
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Red Cabbage Indicator Aim – To create a pH indicator out of a red cabbage solution and to construct a basic pH scale to determine the pH of unknown solutions. Materials - • red cabbage leaves • 250 mL beaker • hotplate or Bunsen burner‚ tripod‚ gauze mat and bench mat • 10 test-tubes – equal size • test-tube rack Methods - Part A: Making the indicator 1. Tear up one or two red cabbage leaves‚ and place them in the beaker with enough water so that the cabbage is just covered. 2. Heat the beaker
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Enzymes INTRODUCTION Enzymes are protein cells and they are found in the gut. They help to break down the food you eat. Enzymes are produced by living cells and they speed up reactions because they are biological catalysts. If we did not have enzymes‚ the reactions in our cells would be too slow to keep us alive. This means that they are extremely important for our survival. The properties of enzymes are: ⋆ They can be used many times‚ because they can speed up reactions without
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the effects of an incorrect pH level. pH measures the concentration of hydronium ions and can be modeled by the function p(t) = −log10t. The variable t represents the amount of hydronium ions; p(t) gives the resulting pH level. Water at 25 degrees Celsius has a pH of 7. Anything that has a pH less than 7 is called acidic‚ a pH above 7 is basic‚ or alkaline. Seawater has a pH just more than 8‚ whereas lemonade has a pH of approximately 3. 1) Create a graph of the pH function either by hand or using
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