Polyacrylamide Gel Electrophoresis (SDS-PAGE) was conducted using a negatively charged protein and pre-stained molecular weight markers. The hypothesis was that the molecular weight of N-acetyl-β-D-hexosaminidase B would be 28‚000 kDa. To confirm or reject the hypothesis‚ the molecular weight of N-acetyl-β-D-hexosaminidase B and the concentration of protein had to be determined. The electrophoresis of the protein gel were conducted using a Hoefer SE 600 Ruby at 30 mA and stained with Coomassie Brilliant
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An enzyme is typically a protein with a specific three-dimensional shape. As previously mentioned above a small part of this shape forms the active site‚ where the enzyme combines with the substrate. The substrate actually fits into the active site‚ which is why enzymes are specific
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Four environmental factors of enzymes were tested in lab. The changing of pH‚ substrate concentrations‚ temperature‚ and an inhibitor (NaCl) and the effects it hade on the enzyme turnip peroxidase. Enzymes are biological catalysts which increase reaction rates by lowering the activation energies of substrates. A substrate is a reactant that interacts with the enzyme. The enzyme and substrate can be viewed as the recently discovered "induced fit model"‚ which suggests enzymes are flexible and dynamic
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the process by which a liquid is separated from a gel owing to further coagulation Saturated fat: It comes mainly from animal food products at a normal room temperature. HDL cholesterol: Lipoproteins‚ which are combinations of lipids (fats) and proteins‚ are the form in which lipids are transported in the blood. HDL cholesterol is considered the "good" cholesterol. The higher the HDL cholesterol level‚ the lower the risk of coronary artery disease. Triangle test- A sensory-based analysis where
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the membrane. B. Small enough to pass through the spaces between the phospholipid molecules. C. Requires the assistance of transport proteins. ______ Nonpolar materials ______ Water ______ Ions ______ Large polar uncharged molecules ______ Small polar uncharged molecules Cell Unit Activity #5 page 6 2. What is the role of transport proteins in biological membranes? _____________________________________________________________ _____________________________________________________________
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TITLE Homeostasis –Urine Analysis OBJECTIVES 1) To know know the various urine components 2) To detect abnormal constituents 3) To get a brief introduction on clinical biochemistry INTRODUCTION Homeostasis mechanism of human body will regulate the osmotic pressure of the blood. This function is carried out by the kidneys‚ that will filter blood and produces urine through three stages: ultrafiltration‚ reabsorption and secretion. The end product of the process will produce urine
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It is important for nurses to assist in the healing of pressure ulcers. Apart from providing appropriate wound dressing‚ various nursing interventions are needed such as relieving pressure by providing special pads or air mattresses and turning or repositioning the patient at least every two hours. Arranging pillows under the ankles‚ back‚ head and arms‚ regular cleansing and drying of the skin‚ and providing good nutrition to support the healing process are also important (Cohen‚ 2009). Optimal
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Polymers-the largest of the macromolecules. These are composed of numerous‚ small identical subunits known as Monomers. There are 4 major polymers that are important for living organisms. These polymers are; carbohydrates‚ lipids‚ proteins and nucleic acids. B. Polymers are referred to as being organic compounds. Organic compounds are compounds that contain the elements carbon and hydrogen. 1. Chemical Properties of Carbon a. Carbon has 6 electrons. What is the structure
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shape of the tertiary structure is three dimensional due to the coils and pleats folding in on themselves when the protein is formed. The tertiary structure is held in place by lots of different types of bonds. A protein’s tertiary structure is vital to its function; one example would be when a hormone must fit a special type of hormone receptor of a target cell. When you heat a protein‚ the kinetic energy increase in the molecule. This causes the molecule to vibrate and breaks some of the bonds holding
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shown to retain their native functional forms. Edible plant vaccine against diarrhoea‚ expressed in potato‚ and antibody against dental caries‚ expressed in tobacco‚ is already in pre-clinical human trials. Attempts are being made to express many proteins of immunotherapeutic use at high levels in plants and to use them as bio-reactors of the modern era. MOST of the drugs used by man‚ until very recently‚ were being derived from plants‚ which subsequently led to pharmaceutical companies starting
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