"Quantification of protein concentration with spectrophotometry" Essays and Research Papers

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    Diffusion is the movement of particles from an area of higher concentration to an area of lower concentration. Because of the selective permeability of the membrane only water and other very small particles (iodine) can be diffused through simple diffusion. The solution out of the dialysis tubing had a higher distelled iodine concentration of solutes (iodine + H2O) than did the starch solution. So iodine move into the cell and react with starch molecules to formed starchiodine‚ the blue compound

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    “Use of Biuret Reagent to Test for Proteins In Food” Cindy Zhou Introduction Protein is a series of amino acids that are connected together by peptide bond. The bond is located between the end of previous amino acid‚ which is the carboxyl group‚ and the head of next amino acid‚ which is a amino group. The Biuret reagent is a solution of copper sulfate and potassium hydroxide. When peptide bond are presented‚ the copper (II) will interact with them (MadSci Network: General Biology). It is

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    Purpose: To find the percent of sugar by mass in chewing gum Hypothesis: The percentage of chewing gum that is sugar for bubble gum is 30%‚ 20% for juicy fruit and 25% for stride gum. Mass Of Juicy Fruit Mass Of Bubble Gum Mass of Juicy Fruit unchewed | 7.12g- 1.72 = 5.4 g | 5.80g- 1.72g = 4.08 | 4.52g- 1.72g = 2.80g | chewed | 3.00g- 1.72g= 1.28g | 2.24g-1.72g =0.52g | 2.86g-1.72g= 1.14g | Percent of Sugar | 3.00g x 100/ 5.4 = 55.5%

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    The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature

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    Their Eyes Were Watching God Reading Questions Chapter One 1. Describe the contrast made for the “porch sitters” as workers and as storytellers (1-2). 2. How do the porch sitters respond to Janie’s return to town? • Men • Women 3. What is Janie’s impression of the porch sitters? Chapter Two 1. Janie has an identity problem until she is around six. Why? • racial identity problem • personal identity problem • social identity problem 2. On page 12‚

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    Investigation - Solution Colour and Ion Concentration Aim: To investigate whether solution colour can be used to reliably determine the concentration of coloured ions in a solution. Hypothesis: the concentration of permanganate ions in the solution is inversely proportional to the percentage transmission of light through the solution. Dependent variable: concentration of permanganate ions. Independent variable: percentage transmission of light through the solution. Equipment: 20 ml

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    Fish phylogenetic tree based on protein size Amanda Reed Lab partners: Lecia Redwine‚ Kyle Hatcher TA: Baneshwar Singh Biology 117‚ Section 25 Tuesday 10:30 AM Introduction       Although tree diagrams have been used since the days of Charles Darwin‚ biologists have only recently adopted the tree model of evolution to read and interpret phylogenies. One of the reasons for this is the confusion that often arises from using a tree model to describe a phylogeny (Baum‚ 2008a). Many people interpret

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    For the 20%‚ the change in temperature was completed by around the 3rd-minute mark‚ so relatively fast before plateauing at 25 degrees. But the 3% took almost the whole 7 minutes to finish rising in temperature. So essentially‚ the higher the concentration of hydrogen peroxide‚ the faster the reaction will take place. Furthermore‚ the foam (water and oxygen produced by the potatoes catalase reacting with hydrogen peroxide) was a kind of indicator of how the reaction was going. It almost indirectly

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    In this folio task‚ the impact of drug absorption and blood stream concentration‚ on the human body‚ will be observed. The rate of absorption and clearance of the drug‚ relies on many factors. The drugs blood stream concentration‚ time of administration‚ absorption rate‚ clearance rate and the effect of certain parameters‚ will be calculated. This can be completed through using a surge function‚ calculus (derivatives) and straight line graphs (). Graphing technology on the graphics calculator

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    13‚ 2013 Determining the Concentrations of Red Dye in Sodas Abstract The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer‚ we measured how much light is absorbed by various known concentrations of red dye. After collecting this data‚ a standard curve was made that correlated the concentration of red dye #40 to its absorbance

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