Paperose Lab- Introduction to Enzymes Ms. Kim/Honors Biology Purpose To Determine the rate of an enzyme reaction using a “modeled” enzyme and sugar‚ paperase and paperose. Introduction In this lab‚ your hands are the enzyme‚ paperase. This enzyme split the sugar‚ paperose‚ into subunit A and subunit B. You will split this molecule by ripping the paper model down the middle. Materials Paperose models‚ scissors‚ plastic bag‚ container (large plastic cup)‚ stopwatch‚ Procedure 1. Each
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developing opacity of the reaction system to do a kinetics study. A series of acidic thiosulfate solutions of the same volume‚ contained in identical Titertek wells‚ are prepared and placed on a piece of white paper clearly numbered. The decompositions are timed. While the reaction proceeds‚ the number can be clearly seen through the solution. Eventually‚ precipitated sulfur renders to solution opaque‚ the number become invisible‚ and the timing stops. After several runs‚ reaction times are correlated
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My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we
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This assignment discusses chemical kinetics by determining the rate and average value for rate constant based on experimental values given. The orders of the reactants can only be determined experimentally‚ so we must look at how the concentration of the reactants affect the instantaneous initial rate while holding the concentration of the other reactant constant. In this reaction‚ the overall reaction order for the rate law is third because the order for A is second and the order for B is first
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in temperature in the reaction of catechol and catecholase will not change the absorbance of reaction over time. What was your alternative hypothesis? be specific As the environment and temperature is changed from 0 °c to 20°c to 95°c‚ the absorbance of catechol and catecholase reaction will increase over time. The rate of reaction increases as the temperature increases. Results: What were the main trends or patterns in your data (Results)? In the Icebath reaction at 0°c has a mean concentration
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pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction temperature of amylase solution and starch solution increase‚ the reaction rate of amylase and starch will increase. After reach the optimal temperature of amylase‚ the reaction rate of amylase and starch will rapidly decrease. The lock and key model explains my hypothesis
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study of rates of chemical reactions and the mechanisms by which they occur. • The reaction rate is the increase in concentration of a product per unit time or decrease in concentration of a reactant per unit time. • A reaction mechanism is the series of molecular steps by which a reaction occurs. 12/13/2011 mov 2 1 13/12/2011 Reaction rate 2 NO2(g) → 2 NO(g) + O2(g) 12/13/2011 mov 3 Thermodynamic vs kinetics of reaction • Thermodynamics determines if a reaction can occur
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Chemical kinetics‚ also known as reaction kinetics‚ is the study of rates of chemical processes. Chemical kinetics includes investigations of how different experimental conditions can influence the speed of a chemical reaction and yield information about the reaction’s mechanism and transition states‚ as well as the construction of mathematical models that can describe the characteristics of a chemical reaction. In 1864‚ Peter Waage and Cato Guldberg pioneered the development of chemical kinetics
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graphs‚ and calculate rate constants. By determining the number of drops of sodium hypochlorite necessary to make a diluted food dye change to colorless in less than three minutes helps calculate the absorbance which can then be analyzed to find the pseudo rate constant and eventually leading to the rate constant and the rate law. The results show that the m and n are both 1st order which makes the overall order of the blue dye 2nd order. Results: In this experiment‚ rate laws and graphs help calculate
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Mapua Institute of Technology Organic Chemistry Laboratory 2 Final Report Factors Affecting the Relative Rates of Electrophilic Aromatic Substitution Reaction Justiniano‚ Priscilla Raiza N. School of Chemical Engineering and Chemistry‚ Mapua Institute of Technology‚ Intramuros‚ Manila‚ Philippines Experiment No.1‚ Submitted on August 6‚ 2011 at N402. Abstract EXPERIMENT NUMBER ONE IS ALL ABOUT THE ELECTROPHILIC SUBSTITUTION OF AROMATIC COMPOUNDS. AROMATIC COMPOUNDS ARE THOSE ORGANIC
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