Introduction Methionine and cysteine are both sulphur containing amino acids. Most proteins will contain one‚ or both of them at some point in the polypeptide chain. As such‚ many amino acids contain sulphur in some form‚ which is required in small amounts in the mammalian diet. Methionine has a thioether side chain‚ and cysteine’s contains a thiol group. These side chains exist as free thiols inside the cell‚ and are oxidised causing them to pair up and form disulphide bonds in an extracellular
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Isolation of Recombinant Taq Polymerase for PCR Isolation of Recombinant Escherichia coli IPTG induced Taq polymerase and characterization through polymerase chain reactions‚ Western Blotting and gel electrophoresis * Braeden Cowbrough1‚ Michael Atkins2‚ Christopher Bonner3 From the Faculty of Biochemistry Lab 3006 B Carleton University‚ Ottawa‚ ON K1S 5B6 *Running title: Isolation of Recombinant Taq Polymerase for PCR To whom correspondence should be addressed: Braeden Cowbrough‚ Faculty of Biochemistry
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Effects of long-term ketamine administration on rat bladder protein levels: A proteomic investigation using two-dimensional difference gel electrophoresis system Abbreviations & Acronyms 2D-DIGE = two-dimensional difference gel electrophoresis CHAPS = 3-[(3Cholamidopropyl) dimethylammonio] propanesulfonate Cy = cyanine dye DIGE = difference gel electrophoresis DTT = DL-Dithiothreitol GAPDH = glyceraldehyde 3-phosphate dehydrogenase HK = high dose of ketamine IC = interstitial
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Title of Experiment Extraction of Spinach Date that the Experiment was Performed This experiment was performed on Wednesday‚ September 17th‚ 2014 at 2:45 pm in the St Ignatius Science Center Laboratory 323. Partners Names Taylor Jackson and Matt D’Angelo. Taylor‚ Matt‚ and I shared the same data. Purpose/Goals/Objectives The purpose of this experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to
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water from a colloidal silica gel without collapse its overall structure. When gels get dry normal temperatures and pressures its affect the surface tension in tiny pores of the gel causes the structure to collapse and reduce itself to roughly 10 times its original volume. To produce aerogel‚ a gel is placed in a vessel of high heat (280°C or 536°F) and pressure (1800 pounds per square inch‚ or 1241 Newton’s per square centimeter). This causes all the liquid within the gel to transform into a supercritical
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. Yes‚ comparably to TLC plate 2 and 3. The benzophenone is more neutral but as the same time its nonpolar‚ being that Silica gel in TLC 2 was polar and the solvent ethyl acetate-hexane is non-polar it moved relatively with the solvent. The stationary phase in TLC 3‚ alumina is non polar and the solvent ethyl acetate is polar so from the ideal that polar does not attract non-polar benzophenone stick stronger to alumina non-polar rather than moving up with polar solvent ethyl acetate as it did in
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Unknown sample: Rf = 2.45 3.00 = 0.82 Discussion: Silica gel is a form of silicon dioxide (silica). The silicon atoms are joined via oxygen atoms in a giant covalent structure. However‚ at the surface of the silica gel‚ the silicon atoms are attached to -OH groups. So‚ at the surface of the silica gel you have Si-O-H bonds instead of Si-O-Si bonds. The diagram shows a small part of the silica surface. [pic] The surface of the silica gel is very polar and‚ because of the -OH groups‚ can form
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polar compound based on structure and when it was mixed with a polar solvent its mobile phase was the slowest‚ therefore‚ it did not travel very far up the silica gel plate. When 9-fluorenol was mixed in slightly polar solvent‚ 10% EtOAc‚ its mobile phase also did not travel very far. The only time 9-fluorenol mobile phase travelled up the silica gel plate is when it was combined with
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Today‚ I’m going to explain how aerogel (otherwise known as frozen smoke) is so unique to any other known materials in the world. An aerogel is a synthetic porous ultra-light material derived from a gel‚ in which the liquid component of the gel has been replaced with a gas. The result is a solid with extremely low density and thermal conductivity. Nicknames include "frozen smoke"‚ "solid smoke"‚ "solid air" or "blue smoke" owing to its translucent nature and the way light scatters in the material;
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reactions to form the porphyrin (your report should discuss the mechanism in great detail‚ including the regiochemistry). The initial porphyrinogen product is not fully aromatic‚ but oxidation from atmospheric oxygen in the presence of the Lewis acid silica gel allows formation of the fully conjugated porphyrin product. Using benzaldehyde as the electrophile will result in a phenyl substituent on each carbon that links the pyrrole units. The porphyrins synthesized in this experiment will not be bound
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