We had started our experiment by learning about the change in the earth and what it does to the concentration in the oceans water. When global changes occur‚ they don’t only change that one thing it changes many things. Such as concentration in the water. For example‚ when heavy machinery or facilities burn fossil fuels‚ which releases more carbon dioxide in the air‚ the ocean sucks it up and the acidity in the water completely changes even if it goes up by .1 or .2 percent it’s still a lot and will
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Task 1c – M3: Glucose Solution Concentration (%) Absorbance (Arbitrary Units) 1 1.61 0.1 0.32 0.01 0.23 0.001 0.22 How to use a Colorimeter: • A Colorimeter measures the absorbance of particular wavelengths of light by a specific solution. • Firstly to use the Colorimeter you must switch it on 5 minutes before use to allow it to stabilize. Next select the most appropriate filter for the analysis and then anter it into the light channel or the selector depending on the type of Colorimeter you
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September 19‚ 2014 Period 4 Lab #1: Density Determinations for Solutions Theory: The density of a sample of matter is very useful when trying to find the identity of an unknown substance. The units of density are quoted in (g/mL) for liquid samples of matter. For that reason if the volume is known of a liquid‚ determining its density is easily determined by weighing it accurately. Density can also be used as a tool for finding the concentration of solutions in some cases. The density is different
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the concentration of Vitamin C in the tablet. Theory: This experiment involves titration of redox reaction. Redox reaction means reduction and oxidation. In which oxidizing agent and reducing agent are mixed and oxidize/reduce each other. One losses electron(s) and increase in oxidation number when is oxidized‚ and vice versa. In order to find out concentration of the ascorbic acid in the tablet‚ a redox reaction of it with iodine was done. While iodine is an oxidizing agent and ascorbic acid
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ESTIMATION OF IRON IN IRON ORE-SPECTROPHOTOMETRIC METHOD By: Taylor Villari Experiment conducted on 7/22/13 Components of each test tube examined in the spectrophotometer Trial | Volume of Iron solution (mL) | Micrograms of Iron | Volume of 10% sodium acetate | Volume of 0.1% o-phenanthroline | Volume of water (mL) | 1(blank) | 0.0 | 0.0 | 1.0 mL | 1.0 mL | 8.0 mL | 2 | 1.0 mL | 10 | 1.0 mL | 1.0 mL | 7.0 mL | 3 | 3.0 mL | 30 | 1.0 mL | 1.0 mL | 5.0 mL | 4 | 5.0 mL | 50 | 1.0 mL
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Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Introduction Why: The goal of this experiment is to determine the water hardness of the prepared unknown water sample. Determining water hardness is a useful test that measures the quality of water for its household and industrial users. Water’s hardness arises from the presence of metal ions – specifically metal ions with a charge of +2 or higher. The ‘scum’ left over in a cleaned shower stall or coffee pot is equivalent to water hardness. Water dissolves salts which contains ions of sodium‚ magnesium
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Investigation - Solution Colour and Ion Concentration Aim: To investigate whether solution colour can be used to reliably determine the concentration of coloured ions in a solution. Hypothesis: the concentration of permanganate ions in the solution is inversely proportional to the percentage transmission of light through the solution. Dependent variable: concentration of permanganate ions. Independent variable: percentage transmission of light through the solution. Equipment: 20 ml
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The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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