To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes
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temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the enzyme to break down the starch solution will occur at a faster rate because
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It about modified starch Journal of Agricultural Science Vol. 2‚ No. 2‚ June 2010 requirements are properties such as viscosity‚ resistant to shear‚ low pH and high temperature. Accordingly‚ varieties of modified starches are used in food industry. Table 1 lists some of the modified starches that can be prepared from different sources to meet the marketing-related requirements. Today‚ modified food starch is a food additive and limits of its modification‚ use and labeling are clearly defined
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The Rate of Reaction that Enzyme Concentration‚ pH‚ and Temperatures Have on the Amylase Enzymes Color Disappearance Abstract: Compare reaction rates of the concentrations‚ pH’s‚ and temperatures of the enzyme Amylase. At what concentrates do the substrate molecules collide with each other‚ making the reaction possible? At what pH levels do the 3D molecular structures change breaking the H-bond and/or denaturize? At what temperatures do the collisions of the substrate molecules happen
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2 Y Nutrition in Animals food into simpler substances is called digestion. You have studied in Class VI that food consists of many components. Try to recall and list them below: 1. ______________________ 2. ______________________ 3. ______________________ 4. ______________________ 5. ______________________ 6. ______________________ no t The components of food such as carbohydrates are complex substances. These complex substances cannot be utilised as such. So they are broken down into
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Fungal Amylase and hydrolysis of Starch Abstract This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates‚ then two drops of the mixture of amylase-starch
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Starch From Wikipedia‚ the free encyclopedia Starch Identifiers CAS number 9005-25-8 EC-number 232-679-6 RTECS number GM5090000 Properties Molecular formula variable Molar mass variable Appearance white powder Density 1.5 g/cm3 Melting point decomp. Solubility in water none Hazards MSDS ICSC 1553 EU Index not listed Autoignition temperature 410 °C (verify) (what is: / ?) Except where noted otherwise‚ data are given for materials in their standard state
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Mechanical digestions starts from the intake of food into the mouth and when the food is chewed and while the food is broken down until it becomes small to be swallowed. Then the food goes down as a food bolus into the esophagus after the food is broken down. The food bolus passes through the alimentary canal through peristaltic movements. Peristaltic movements is a systematic series of muscle contractions and relaxation which involves the passage of food bolus through the esophagus‚ stomach‚ small
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Exercise 8 Chemical and Physical Processes of Digestion NAME Dane Wilson LAB DATE/TIME august 4th Carbohydrate Digestion The following questions refer to Activity 1: Assessing Starch Digestion by Salivary Amylase. 1. At what pH did you see the highest activity of salivary amylase? Why? 7.0 because that is when the salivary is most effective and it breaks down carbohydrates. 2. How do you know that the amylase did not have any contaminating maltose? We used
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hydrolysis of starch is carried out under milder conditions: lower temperatures (up to 1000C)‚ normal pressure‚ pH of the medium around 6 – 8. At the same time enzymatic hydrolysis is characterized by high reaction rate‚ high stability of the enzyme towards the denaturizing action of solvents‚ detergents‚ proteolytic enzymes‚ and a decrease in the viscosity of the reaction medium at higher temperatures‚ etc. Most often‚ enzymatic hydrolysis is carried out with the enzyme α-amylase from different
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