MIC 230 Unknown Identification Report The objective of this experiment was to identify an organism from a mixture of two unknown bacterial species. In order to accomplish this‚ I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully
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Identification of an Unknown Organic Acid Chem 1211K Lab Drawer #15 Wednesday November 13th‚ 2013 Unknown Number: 3334025-CF13 Table of Contents Pg. # Abstract 3 Experimental Report 4-7 Results and Discussion 7-12 Conclusion 13 **pKa Graph 14 Abstract Identifying this organic acid was an extensive task that involved several different experiments. Firstly‚ the melting
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Experiment H: Identification of an Unknown Organic Compound The objective of this lab was straightforward. We were given an unknown compound and we were to perform an IR spectroscopy and as well as NMR spectroscopy. With the IR spectroscopy‚ I was able to name the functional groups I have on my compound and further confirmed my assumptions by looking at the NMR spectroscopy after. The unknown number I was given was number 203. The molecular weight of the compound was 121. From the molecular weight
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Substitution | Synthesis of n-Butyl Bromide and t-Pentyl Chloride | | Jessica | [Pick the date] | Abstract The synthesis of the alkyl halide n-Butyl Bromide from alcohol is the foundation for the experiment. During the isolation of the n-butyl bromide‚ the crude product is washed with sulfuric acid‚ water‚ and sodium bicarbonate to remove any remaining acid or n-butyl alcohol. The primary alkyl halide halide n-butyl bromide is prepared by allowing n-butyl alcohol to react with sodium bromide and sulfuric
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Cyclohexene Synthesis by Dehydration Reaction of Cyclohexanol Gjulia Vokrri 02-15-2013 Abstract Alkenes can be produced by heating and dehydrating an alcohol in the presence of a strong acid. The purpose of this experiment was to synthesize cyclohexene by dehydration of cyclohexanol and to detect the presence of a double bond in the alkene. The dehydration reaction using distillation was
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Grainy and shiny white Becoming all liquid 125 Clear B. Melting point of unknown #42 1st trial Temperature (¡ÆC) Observation Unmelted Shiny light yellow crystal First liquid appear point 99 Grainy yellow Becoming all liquid 101 Clear 2nd trial Temperature (¡ÆC) Observation Unmelted Shiny light yellow crystal First liquid appear point 95 Grainy yellow Becoming all liquid 97 Clear C. Melting point of mixture (Unknown + Benzil) Temperature (¡ÆC) Observation Unmelted Shiny light yellow
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For the unknown light source‚ it had almost every color‚ so it might have been be mercury because they have similar color beams and their color from the naked eye appeared as purple‚ which mercury‚ a light blue‚ is very close to in terms of it’s color on the spectrum. For the unknown flame crystals‚ it may NH4+ because the colors that appeared are very similar to the crystals. Next‚ chemicals have to be heated in the flame for the color to emit because heat adds energy to the substance making the
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A Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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very unique. The hydrogen-ion concentration of biological system is usually preferred as the pH system‚ which determines the pH level of dilute aqueous solutions. In this laboratory‚ the data collected from the experiment will be used to graph the titration curves which help to identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly
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Relative Reactivity of Alkyl Halides Introduction Nucleophilic substitution of alkyl halides can proceed by two different mechanisms – the SN2 and the SN1. The purpose of the experiment was to identify the effects that the alkyl group and the halide-leaving group have on the rates of SN1 reactions‚ and the effect that the solvent has on the rates of SN1 and SN2 reactions. The SN1 mechanism is a two-step nucleophilic substitution‚ or unimolecular displacement. In the first step of the mechanism
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