Factors that Affect the Rate of Reaction of Peroxidase Purpose: To determine the effect of various factors on the rate of reaction between an enzyme and its substrate‚ and also to determine the optimal ranges under which the enzyme activity is maximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range (pH 7) because the
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have no effect on the reaction whatsoever.I predict alcohol is an inhibitor of Peroxidase because alcohol when alcohol bind to theallosteric site it changes the active site shape of the enzymes thus deactivating enzymaticactivitiesI predict salt is an activator of Peroxidase because salt contains Na ions which attaches tothe allosteric site changing the shape of the enzyme to fit a substrate. Materials: • Peroxidase (enzyme in potato) • Hydrogen peroxide‚ 3% • A strong acid‚ pH3 (lemon juice
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Title of Lab: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of
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How Enzymes Work In Different Environments By Sarah Smith Biology1111 October 20‚ 2011 Lab Partner: Nellie Greer ABSTRACT Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide‚ H2O2‚ into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this‚ we added different levels of pH‚ low‚ medium‚ and high‚ into different test tubes with the enzyme and H2O2‚
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and enzyme concentration levels and how it effects the catalyst reaction times of catecholase and peroxidase enzymes using potato extract. A review on how enzymes react under certain external conditions gave a good foundation to base this experiments hypothesis when looking at concentration of enzymes‚ temperature and pH levels.
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which was done by using the equation C₁V₁ = C₂V₂. Other materials consisted of pH 7 buffer‚ guaiacol which was used as our indicator‚ and 1% turnip peroxidase solution. Eight cuvettes were needed‚ four of which were blank tubes which means it did not contain guaiacol‚ the indicator. The other four cuvettes were experimental tubes. The pH 7 buffer‚ peroxidase‚ guaiacol‚ and Hydrogen Peroxide were added into the experimental tubes. The order in which you add these solutions is very important. The Hydrogen
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The reactions and Results of Using Enzyme Turnip Root Peroxidase Lab results from: Andrew Compton‚ Mickey. Results published 9/29/2012. TA: In this series of laboratory experiments‚ my lab partner and I were to conduct an experiment about the oxidation rate of the enzyme peroxidase in the presence of its substrate guiacol. Also we used other substrates‚ such as hydroxylamine an enzyme inhibitor‚ to observe the weather the reaction rate was slowed down‚ sped up‚ or stopped reactions all together
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Interactions BigIdea 4 investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? ■■BACKGROUND Enzymes speed up chemical reactions by lowering activation energy (that is‚ the energy needed for a reaction to begin). In every chemical reaction‚ the starting materials (the substrate(s) in the case of enzymes) can take many different paths to forming products. For each path‚ there is an intermediate or transitional product between reactants and
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each (#1‚ 2‚ 4‚ 9‚ 11‚ and 12) being a substrate with guiacol product indicator‚ and a neutral buffer. The guiacol is a naturally organic compound and is actually a phosphate. The other six test tubes (#3‚ 5‚ 6‚ 7‚ 8‚ and 10) had the enzyme turnip peroxidase and different pH solutions for each of the six test tubes. The different pH levels were 3‚ 5‚ 6‚ 7‚ 8‚ and 10. The lower the pH‚ the more acidic the liquid is. The higher the pH‚ the more basic with alkaline. We mixed the six test tubes with
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Enzyme Activators and Inhibitors Lucia House AP Biology‚ Block 4 Mr. Trice October 18‚ 2012 Introduction: Metabolism is the totality of all of an organism’s chemical reactions. Chemical reactions occur due to enzymes‚ a substance which acts as a catalyst in driving chemical reactions in order to produce a desired product (Campbell and Reece‚ 2002). A catalyst is usually a protein; however‚ some catalytic molecules counter this generalization. A discovery made in the early nineteen- nineties
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