experiment shows the effects of changing the pH level has on Catalase. As predicted‚ the farther away the pH levels got from the optimum pH (7.2)‚ the lower the reaction rate. At a pH of 7.2‚ the foam of the reaction measures 6cm. At a ph of 3 it measures 2.5 cm‚ at a pH of 5 it measures 2.75 cm‚ at ph 9 it measures 2.3 and at 11cm it measures 2cm. pH measures the hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result of altering
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(4) uses of energy in biological processes (B); active transport; muscle contraction; nerve transmission; synthesis; translocation; nitrogen fixation; receptors ENERGY TRANSFERS WHICH TAKE PLACE INSIDE LIVING ORGANISMS Living organisms including all plants and animals require energy for their cellular processes. In biological processes‚ the immediate energy source is often in the form adenosine triphosphate (ATP). The nucleotide ATP maintains both catabolic and anabolic reactions. An example of
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concentrations of catalase are added with hydrogen peroxide. Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of. Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes: Hundreds of chemical reactions happen simultaneously inside living cells and it’s the job of enzymes to control and regulate the various metabolic activities. Enzymes are biochemical catalysts‚ which speed up reactions
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launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach)‚ Brassica oleracea (Broccoli)‚ Solanum tuberosum (Russet Potato)‚ Malus domestica (Apple)‚ and Allium cepa (Onion). The five different catalases from the sources will all be used
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its optimum point of saturation‚ after which any increase in the substrate concentration will no longer affect the rate of reaction. The independent variable in this investigation is the varying concentrations of the substrate (Hydrogen Peroxide: 1%‚ 3%‚ 5% and 6%)The dependent variable was the rate of enzyme catalase activity‚ which was measured by the volume of froth produced after one minute.The concentration and volume of liver extract (source of catalase) used was kept constant at 10ml per solution
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Introduction: Catalase is an enzyme‚ which is present in all life forms that utilize oxygen for their biological processes. Enzymes are proteins which increase the rate of reaction by decreasing the activation energy (the energy required to initiate a reaction). One of the main function of Catalase is to prevent the accumulation of hydrogen peroxide (H2O2) in the body by breaking it down into water and oxygen gas. Hydrogen peroxide is a toxic by-product of metabolic reactions. If hydrogen peroxide
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trend O2 production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment
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table of results. The graph shows that the highest rate of reaction is at pH 7. This therefore supports my theory that at pH 7 the rate of reaction will be highest. This relate to other resources‚ such as books and the internet‚ which show that the optimum pH value for catalase is pH7‚ therefore there is no difference between the optimum pH and the pH used in the experiment‚ hence the enzyme structure is not affected by the pH and is most active. However the graph inadequately supports the second
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Investigation into how pH levels affect Catalase activity in potato samples – (enzymes) Introduction: The aim of this investigation is to understand and monitor the effects pH levels have on catalase activity within controlled potato samples. Catalase is a type of enzyme that is found widespread among organisms that grow in the presence of oxygen (almost all living things) its specific use is to speed up the decomposition of Hydrogen peroxide (H2O2) within cells. Hydrogen peroxide is a bi-product
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The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of
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