We are studying the effect of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure
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with water by using mechanical pressure from the sugarcane. On the other hard the beet is peeled and crushed. Sucrose that was extracted from sugarcane is a highly effective substrate that is used during yeast fermentation (Alexander N.Glazer). An enzyme that breaks down sugar is produced by the yeast . The enzyme is produced in the cytoplasm as well as in a secreted form. This enzyme is a catalyst for the
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produced during this cycle. Temperature and environment can affect the process of fermentation. In this lab‚ we will be measuring the amount of CO2 produced in different temperature. If we have different temperature of yeast (0*‚ 22*‚37* and 100*). The yeast solution will have no activity or very little to measure at 0*. Temperature at 37* would show the most activation or CO2 production. The yeast solution at 100* will show no activity because that high of a temperature will cause denaturing to occur
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RaeAnne Smith HL Biology Y2 Soule: Period 7 10 October 2011 Affect of Sugars on Yeast Respiration Introduction Cellular respiration can be defined as the release of energy‚ or the breakdown of carbohydrates into carbon dioxide and water1. Cell respiration takes place in the mitochondria of animals and in the cytoplasm of plants. The formula for aerobic cellular respiration is: c6H12O6+ 6O2→6CO2+6H2O Aerobic respiration occurs when oxygen is present‚ while anaerobic respiration occurs when
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Name of Student: Bal Krishna Maharjan Course: Food Microbiology 17/FA Describe how to isolate and differentiate between molds and yeasts? Answer: Isolation of molds: Isolation of molds can be done in two ways: 1. Direct isolation: Under this different methods can be selected depending upon the nature of molds and need of study. The following are the different direct isolation techniques. a. Direct transfer: Involves simple transfer of a mold from its source to a pure culture‚ usually consists of
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tested the effect of temperature on the rate of fermentation in yeast by measuring the height of CO2 produced in a graduated cylinder at varying degrees Celsius. Yeast can perform cellular respiration and fermentation‚ the first needing oxygen (aerobic) and the second not (anaerobic). Cellular respiration is the normal way human bodies’ make energy‚ but when lacking oxygen‚ cells undergo fermentation‚ which creates less energy than respiration‚ CO2‚ and lactic acid (though in yeast‚ ethel alcohol is
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data. As evident in the table‚ the 10oC temperature is very accurate as the range was 0‚ resulting in an error percentage of 0%. However‚ the 35oC temperature had a no clear skew of results as indicated through the range‚ 14 and the error percentage‚ 161.47%‚ suggesting that the trials at this temperature were not very accurate as they were largely inconsistent. The 50oC also proved to have inaccuracies‚ however‚ not as significant as the previous temperature‚
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concentrated‚ which was hard to identify the shape of the bacteria‚ while other sections were less concentrated and was easier to analyze the shape. The next step according to the result‚ will be a catalase test.
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The Effect of Yeast on Different Fruit Juices | Researched by Kristyn S. 2001-02 | * PURPOSE * HYPOTHESIS * EXPERIMENT DESIGN * MATERIALS * PROCEDURES * RESULTS * CONCLUSION * RESEARCH REPORT * BIBLIOGRAPHY * ACKNOWLEDGEMENTS * ABOUT THE AUTHOR | PURPOSEThe purpose of this experiment was to determine the amount of fermentation of four different fruit juices after adding yeast. I became interested in this idea when I saw the fruit in my family’s refrigerator
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Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide
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