Anticancer Drug Development : Evaluation of Anti-Proliferative Activity of the Seeds of Dicerocaryum Seneciodes on Leukemia T Cell Line.
Evaluation of the phytochemical composition and the anti-proliferative potential of seed extract of Dicerocaryum senecioides on Jurkat T cell lines By Humbulani Ronald Tshilongamulenzhe A Dissertation submitted to the Faculty of Science and Agriculture, Department of Biochemistry, Microbiology and Biotechnology, School of Molecular and Life Sciences in accordance with the requirements of the University of Limpopo for honours for the degree Bachelor of Science in Biochemistry
UNIVERSITY OF LIMPOPO SOVENGA 0727 SOUTH AFRICA
Supervisor: Prof L.J. Mampuru
NOVEMBER 2011
DECLARATION I declare that the work presented in this dissertation submitted to the University of Limpopo for the degree of Honours in Biochemistry, is my own in design and execution and all information contained herein has been accordingly acknowledged.
Tshilongamulenzhe Humbulani Ronald Student no. 200810649
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DEDICATION I dedicate this work to my parents, Mashudu and Agnes Tshilongamulenzhe, my sister (Mukovhe), my aunt (Regina), my uncles (Titus and Mphelekedzeni), my grandmothers (Johanna and Muofhe) and my pastors (Mr and Mrs Tovhakale) who contributed to my success.
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ACKNOWLEDGEMENTS Firstly, I would like to give thanks to the Almighty God for strengthening me from the beginning of this work until its completion. For it was by His Grace which made it possible for me to pull through this endeavour. I wish to express my heartfelt appreciation to my supervisor, Prof L.J. Mampuru for his guidance, support, suggestions, and encouragement throughout the course of this research. Thank you for trusting and teaching me on how to be self-governing. Special thanks are extended also to different individuals most especially Dr K.C Lucas who strengthened and gave me the guidance before I began with the research. To Dr Mbazima, Dr Mokgotho, Dr T.M Matsibatlela, Ms Pilane and Ms Ntini, thank you for teaching me how to handle the cells. I remembered Dr Mbazima and Dr Mokgotho saying I should handle
UNIVERSITY OF LIMPOPO SOVENGA 0727 SOUTH AFRICA
Supervisor: Prof L.J. Mampuru
NOVEMBER 2011
DECLARATION I declare that the work presented in this dissertation submitted to the University of Limpopo for the degree of Honours in Biochemistry, is my own in design and execution and all information contained herein has been accordingly acknowledged.
Tshilongamulenzhe Humbulani Ronald Student no. 200810649
ii
DEDICATION I dedicate this work to my parents, Mashudu and Agnes Tshilongamulenzhe, my sister (Mukovhe), my aunt (Regina), my uncles (Titus and Mphelekedzeni), my grandmothers (Johanna and Muofhe) and my pastors (Mr and Mrs Tovhakale) who contributed to my success.
iii
ACKNOWLEDGEMENTS Firstly, I would like to give thanks to the Almighty God for strengthening me from the beginning of this work until its completion. For it was by His Grace which made it possible for me to pull through this endeavour. I wish to express my heartfelt appreciation to my supervisor, Prof L.J. Mampuru for his guidance, support, suggestions, and encouragement throughout the course of this research. Thank you for trusting and teaching me on how to be self-governing. Special thanks are extended also to different individuals most especially Dr K.C Lucas who strengthened and gave me the guidance before I began with the research. To Dr Mbazima, Dr Mokgotho, Dr T.M Matsibatlela, Ms Pilane and Ms Ntini, thank you for teaching me how to handle the cells. I remembered Dr Mbazima and Dr Mokgotho saying I should handle
References: Ghobrial, L.M., Witzig, T.E and Adjei, A.A. (2005). Targeting apoptosis pathways in cancer therapy. Cancer Journal of Clinicians, 55(3):178-194.