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Bacterial Staining

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Bacterial Staining
STAINING FOR
BACTERIAL ISOLATION

Submitted by: Cherry Luz L. Rezaga
Submitted to: Dr. Tomas J. Fernandez Jr.

CRITERIA OF REPORTING
1. Thorough research (20%)
1.1. Number of literature cited more than five (5)
1.2. Completeness of the topic (5)
1.3. Principle discussed (5)
1.4. Literature should be cited in the text (5)
2. Confidence (20%)
2.1. Eye to eye contact (5)
2.2. No reading (5)
2.3. Proper handling of visual aids (5)
2.4. Magnified voice (5)
3. Appropriateness of visual aid (15%)
3.1. Appropriateness of visual aids (2.5)
3.2. Clarity of pictures, figures, etc (2.5)
3.3. Readable from a distance of 1.5 meters (2.5)
3.4. Appropriate use of color (not glaring) (2.5)
3.5. Correctness of words spelled (2.5)
3.6. Slide not crowded (2.5)
4. Knowledge of the subjected matter (15%)
4.1. Thorough explanation of subject matter (5)
4.2. Ability to answer questions (5)
4.3. Only relevant topics discussed/included (5)
5. Grammar (20%)
5.1. Organization of thoughts (5)
5.2. Coherence of paragraph (5)
5.3. Tenses, preparation (grammar) (2.5)
5.4. Syntax/Choice of words (2.5)
5.5. Sentences translated in the way it is understood (5)
6. Promptness of submitting report (5%)
6.1. Main criteria written on a ¼ sheet of paper attached to hard copy of report
(2.5)
6.2. Hard copy & soft copy submitted(2.5)
7. Uniform (5%)
7.1. Complete uniform (5)
Total

INTRODUCTION
Bacterial cells are usually colorless because cytoplasm, for the most part, is transparent.
Staining the organisms makes them contrast in color with their surroundings, so they are more readily visible. Certain stains can also be used to identify internal structures of the cell, which would otherwise be unseen. Further, in order to use the oil immersion objective of the microscope and thereby obtain the greatest degree of magnification, it is convenient to use stained preparations rather than wet mounts.1
Although bacteria do not appear greatly different from their

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