1.) Archaea and Bacteria are the oldest, structurally simplest and most abundant forms of life.
2.) Name and describe seven ways in which prokaryotes differ substantially from eukaryotes?
Unicellularity- fundamentally single-celled
Cell Size- can vary (large range changes with species)
Nucleoid- lack a membrane-bound nucleus but rather a nucleoid region
Cell Division/Genetic Recombination- binary fission (does not use spindle) and do not have a sexual cycle
Internal Compartmentalization- does not have internal compartments and no membrane organelles
Flagella- no 9+2 structure
Metabolic Diversity- photosynthesis (oxygenic/produce oxygen and anoxygenic/nonoxygen producing)
3.) Explain 4 ways in which Archaea and Bacteria differ?
Plasma Membrane- ether bond (lipid) can also have branches (tetraethers)
Cell Wall- lack peptidoglycan, has psuedomurein, does the same as peptidoglycan
DNA Replication- similar to eukaryotes
Gene expression- may have two RNA polymers and different than bacteria similar to eukaryotes
4.) Name 5 key characteristics once used in classifying prokaryotes.
Photosynthetic/nonphotosynthetic
Motile/nonmotile
Unicellular/colony-forming/filamentous
Formation of spores/Division by transverse binary fission
Importance as human pathogens or not
5.) Name the domains of life.
Archaea
Bacteria
Eukarya
6.) Name and describe 3 basic forms of prokaryotes.
Bacillus (rod-shaped)
Coccus (spherical/ovoid-shaped)
Spirillum (long and helical-shaped)
7.) The bacterial cell wall is the single most important contributor to cell shape.
8.) Prokaryotic cell walls are constructed of many layers, which is formed from peptidoglycan polymers cross-linked by peptide side chains.
9.) Describe a Gram Stain.
Staining technique that divides bacteria into gram-negative or gram-positive based on retention of a violet dye
10.) Distinguish between gram positive and gram negative bacteria.
Gram-positive- has a