INTRODUCTION
Background of the Study There are lots of herbal plants which are already known for its uses. Binayuyu (Antidesma ghaesembilla Gaertn var. ghaesembilla) is a herbal plant used by B’laan tribes in the southern part of the Philippines. It is said that it can cure stomach ache and helps to lower blood sugar. Artemia is a genus of aquatic crustaceans known as brine shrimp. Artemia, the only genus in the family Artemiidae, has changed little externally since the Triassic period. The historical record of the existence of Artemia dates back to 982 from Urimia Lake, Iran, although the first unambiguous record are the report and drawings made by Schlosser in 1756 of animals from Lymington, England. Artemia are able to avoid cohabitating with most types of predators, such as fish, by their ability to live in waters of very high salinity up to 250%. A method utilizing Brine Shrimp (Artemia salina) has been used to screen medicinal plants for biological activity. Binayuyo (Antidesma ghaesembilla Gaertn var. ghaesembilla) are abundant in the mountains of Malapatan, Sarangani Province and their ethnobotanical uses obtained from traditional healers. Biological activity using the Brine Shrimp bioassay was recorded as a lethal concentration (LC50) that kills 50% of larvae within 24 hours of contact with the Binayuyu (Antidesma ghaesembilla Gaertn var. ghaesembilla) crude extract. The Brine Shrimp lethality bioassay was found to be reliable, inexpensive, and a convenient method for assessment of biologically active medicinal plants. In this research, the researchers will determine the mortality rate as exposed to Binayuyu (Antidesma ghaesembilla Gaertn var. ghaesembilla) bark crude extract by using brine shrimps (Artemia salina) and to determine the total resistance of brine shrimp in binayuyu (Antidesma ghaesembilla Gaertn var. ghaesembilla) crude extract.
Objectives of the Study
Generally this study aimed to determine the mortality rate of