Enzyme Activity Lab Report
Factors Affecting Enzyme Activity
Abstract:
In the following lab factors affecting enzyme activity, temperature, pH, enzyme concentration, substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the rate of reaction will be recorded to see the difference in each factor. In conclusion you will learn whether each factor effects enzyme activity or not.
Introduction:
In the lab: Factors Affecting Enzyme Activity, the objective was to analyze …show more content…
When enzymes catalyze it will either break or build chemical bonds. (Jalink /2014 September 16/ Enzymes note conducted in class at RND). It is predicted that as temperature increases the rate of reaction will increase as well, since kinetic energy causes random collisions between substrate molecules. When the temperature is warm (room temperature) there will be a low rate of reaction since the temperature would not be causing any bond breaking between the substrate molecules. In cold temperature it is predicted that the rate of reaction will decrease because it can cause the enzyme to break apart in their bonds and eventually denature. (aleve1notes). For pH it is predicted that the higher the concentration of acidity or base the higher the chance of the enzyme to denature. The level of acidity or base can effect the structure and effectiveness of the enzyme. Since we will be testing the enzyme with just one level of both acid and base the pH paper will help identify the level of pH that the enzyme will be dealing with. It is predicted that increasing the size of enzyme concentration will also increase the rate of reaction, since …show more content…
Materials:
Beakers
6+ test tubes
1 molar HCl
1 molar NaOH fresh enzyme (cow liver) hot plate distilled water ruler stop watch knife/ incisor pipette liquid soap ice pH paper test tube holder hydrogen peroxide found in stores tweezers scissors
0.5%, 1%, 3%, 5% hydrogen peroxide clamb crusher
Erlenmeyer flask
Abstract:
In the following lab factors affecting enzyme activity, temperature, pH, enzyme concentration, substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the rate of reaction will be recorded to see the difference in each factor. In conclusion you will learn whether each factor effects enzyme activity or not.
Introduction:
In the lab: Factors Affecting Enzyme Activity, the objective was to analyze …show more content…
When enzymes catalyze it will either break or build chemical bonds. (Jalink /2014 September 16/ Enzymes note conducted in class at RND). It is predicted that as temperature increases the rate of reaction will increase as well, since kinetic energy causes random collisions between substrate molecules. When the temperature is warm (room temperature) there will be a low rate of reaction since the temperature would not be causing any bond breaking between the substrate molecules. In cold temperature it is predicted that the rate of reaction will decrease because it can cause the enzyme to break apart in their bonds and eventually denature. (aleve1notes). For pH it is predicted that the higher the concentration of acidity or base the higher the chance of the enzyme to denature. The level of acidity or base can effect the structure and effectiveness of the enzyme. Since we will be testing the enzyme with just one level of both acid and base the pH paper will help identify the level of pH that the enzyme will be dealing with. It is predicted that increasing the size of enzyme concentration will also increase the rate of reaction, since …show more content…
Materials:
Beakers
6+ test tubes
1 molar HCl
1 molar NaOH fresh enzyme (cow liver) hot plate distilled water ruler stop watch knife/ incisor pipette liquid soap ice pH paper test tube holder hydrogen peroxide found in stores tweezers scissors
0.5%, 1%, 3%, 5% hydrogen peroxide clamb crusher
Erlenmeyer flask