Gel Electrophoresis Adventure Lab Report
Gel Electrophoresis Adventure
Intro
The final goal of this lab was to successfully measure the size of different samples of
DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. The DNA samples will move through the gel towards the positive charge.
Ideally, the DNA will move and create and sequence of smallest to largest. This lab exposes us to DNA technology. Backround
Gel electrophoresis is used to separate macromolecules like DNA or RNA by size or proteins by charge. To examine DNA and RNA, the fragments are placed in the agarose wells and an electrical charge is sent through, pushing the negatively charged molecules towards the positive side. The smaller the molecule, the less resistance it will face when hitting the …show more content…
These ends can connect to an identical sequence cut by the same restriction enzymes or a very similar end. Blunt ends are created when a restriction enzyme cuts the double helix evenly.
Materials
One will need buffer solution, pipettes, an electrophoresis chamber, agarose, and three DNA samples consisting of an uncut sample, and a sample cut with EcoRI and one cut with HindIII to complete this lab. Methods
To start things off, the gel must be created. The mold has two open ends, therefore must be taped tightly and repetitively. After pouring the agarose liquid into the mold, it is mandatory that a ‘comb’ is placed in the mold to create the wells as the liquid solidifies. After
20 minutes, it has solidified, remove the comb and the tap and place the gel in the chamber.
The buffer solution is used to deliver the current to the agarose gel. Pour the buffer solution so it covers the gel. Add one of each sample of DNA to separate wells using a pipette. Cover the chamber and make sure the negative side of the circuit is on the same side as the wells.
After two hours of sitting in the electricity, remove the gel and stain it. Rinse the gel thoroughly and let it sit in water for a day.
Intro
The final goal of this lab was to successfully measure the size of different samples of
DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. The DNA samples will move through the gel towards the positive charge.
Ideally, the DNA will move and create and sequence of smallest to largest. This lab exposes us to DNA technology. Backround
Gel electrophoresis is used to separate macromolecules like DNA or RNA by size or proteins by charge. To examine DNA and RNA, the fragments are placed in the agarose wells and an electrical charge is sent through, pushing the negatively charged molecules towards the positive side. The smaller the molecule, the less resistance it will face when hitting the …show more content…
These ends can connect to an identical sequence cut by the same restriction enzymes or a very similar end. Blunt ends are created when a restriction enzyme cuts the double helix evenly.
Materials
One will need buffer solution, pipettes, an electrophoresis chamber, agarose, and three DNA samples consisting of an uncut sample, and a sample cut with EcoRI and one cut with HindIII to complete this lab. Methods
To start things off, the gel must be created. The mold has two open ends, therefore must be taped tightly and repetitively. After pouring the agarose liquid into the mold, it is mandatory that a ‘comb’ is placed in the mold to create the wells as the liquid solidifies. After
20 minutes, it has solidified, remove the comb and the tap and place the gel in the chamber.
The buffer solution is used to deliver the current to the agarose gel. Pour the buffer solution so it covers the gel. Add one of each sample of DNA to separate wells using a pipette. Cover the chamber and make sure the negative side of the circuit is on the same side as the wells.
After two hours of sitting in the electricity, remove the gel and stain it. Rinse the gel thoroughly and let it sit in water for a day.