Protein Concentration
In this report, the concentration of total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum, a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein, it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol green (BCG) reagent. When albumin and the BCG reagent are combined, the solution turns into a blue-green complex. Albumin and globulin are protein components in the total protein; the total protein of blood plasma is composed of 40-60% of albumin. According to the laboratory handout, albumin is responsible of binding and transporting cations, fatty acids, hormones, water and pharmaceuticals throughout the
…show more content…
In five of the test tubes, different concentration of protein sample (10 ul) were added into them; the absorbance of these standards were used for the standard curve graph. The absorbance of the control, patient 1, and patient 2 were measured by the UV-Visible Spectrometer at a wavelength of 637 nm.
Results and Discussion
The concentration of total protein of the control and patients, shown in Table 1, were calculated by using the y= mx+ b from the absorbance vs. concentration graph (Figure 1). An example of the calculation is shown below.
Control (C1): Total Protein Concentration Calculation
The Y value of the equation is the absorbance and the X value is the concentration; therefore, the y= mx+ b equation was rewritten. y= mx+ b x= (y-b)/m
The linear equation of Figure 1 is y= 0.0189x+ 0.0093 and the absorbance of the control was 0.164, thus: x= (0.164-0.0093)/0.0189= 8.19 g/L
Table 1: Total Protein: Absorbance and Concentration
Tube Absorbance Concentration (g/L)
Saline 0
In five of the test tubes, different concentration of protein sample (10 ul) were added into them; the absorbance of these standards were used for the standard curve graph. The absorbance of the control, patient 1, and patient 2 were measured by the UV-Visible Spectrometer at a wavelength of 637 nm.
Results and Discussion
The concentration of total protein of the control and patients, shown in Table 1, were calculated by using the y= mx+ b from the absorbance vs. concentration graph (Figure 1). An example of the calculation is shown below.
Control (C1): Total Protein Concentration Calculation
The Y value of the equation is the absorbance and the X value is the concentration; therefore, the y= mx+ b equation was rewritten. y= mx+ b x= (y-b)/m
The linear equation of Figure 1 is y= 0.0189x+ 0.0093 and the absorbance of the control was 0.164, thus: x= (0.164-0.0093)/0.0189= 8.19 g/L
Table 1: Total Protein: Absorbance and Concentration
Tube Absorbance Concentration (g/L)
Saline 0