Minimum Inhibitory Concentration Lab Report
A protein standard curve was generated by using the protein standard (1 ampule) measurement at 595 nm. Standard curve was prepared as shown in Table 4.
Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively.
3.10.2 Protein Determination
The treated and untreated samples were measured using Bradford assay. 10µl of the samples were made up to 100µl with distilled water. 30µl of the diluted samples was mixed with 1.5ml of Bradford reagent and incubated for 10 minutes before measured …show more content…
S.aureus (ATCC 25923) with overnight incubation at 37°C (Figure 2). The stock extract was diluted with 10% DMSO. MIC was observed as the lowest concentration of extract showing no visible growth in test tube. The MIC value of the methanolic extract of A.paniculata was 0.06mg/ml (Table 2).
4.3 Minimum Bactericidal Concentration (MBC)
Minimum bactericidal concentration (MBC) was performed to determine the loweest concentration of the extract that killed the bacteria. After the value of MIC was recorded, one loop of each concentration dilution were subcultured on BHI agar and incubated at 37°C overnight (Table 3). The MBC of methanolic extract of A.paniculata was 0.06mg/ml (Figure 3).
4.4 Time-Kill Assay
Time-kill assay was carried out with S.aureus isolated. Viable cells counts of the bacteria after treatment with A.paniculata extract were decreased within hours of incubation at 37°C. The result of time-kill assay was shown in Figure 4a, Figure 4b, Figure 4c, Figure 4d and Figure 4e. The growth control and organism control showed the highest number of cfu/ml whereas for extract control there was no growth of
Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively.
3.10.2 Protein Determination
The treated and untreated samples were measured using Bradford assay. 10µl of the samples were made up to 100µl with distilled water. 30µl of the diluted samples was mixed with 1.5ml of Bradford reagent and incubated for 10 minutes before measured …show more content…
S.aureus (ATCC 25923) with overnight incubation at 37°C (Figure 2). The stock extract was diluted with 10% DMSO. MIC was observed as the lowest concentration of extract showing no visible growth in test tube. The MIC value of the methanolic extract of A.paniculata was 0.06mg/ml (Table 2).
4.3 Minimum Bactericidal Concentration (MBC)
Minimum bactericidal concentration (MBC) was performed to determine the loweest concentration of the extract that killed the bacteria. After the value of MIC was recorded, one loop of each concentration dilution were subcultured on BHI agar and incubated at 37°C overnight (Table 3). The MBC of methanolic extract of A.paniculata was 0.06mg/ml (Figure 3).
4.4 Time-Kill Assay
Time-kill assay was carried out with S.aureus isolated. Viable cells counts of the bacteria after treatment with A.paniculata extract were decreased within hours of incubation at 37°C. The result of time-kill assay was shown in Figure 4a, Figure 4b, Figure 4c, Figure 4d and Figure 4e. The growth control and organism control showed the highest number of cfu/ml whereas for extract control there was no growth of