Science Invertigatory Project
Bitoon Seeds Extract (Barringtonia asiatica) as Potential Antibacterial Agent
An Investigatory Project Submitted As Entry to the
2010 Regional Science Fair
Jorge Andrew C. Bagaipo
Jessa Mae C. Macana
Loriel Mari E. Berte
Researchers
Ma. Edylyn L. Noguerra
Adviser
ABSTRACT
The study entitles “Bitoon seeds Extract (Barringtonia asiatica) as Potential Antibacterial Agent aims that the antibacterial agent of the seeds and determines its inhibitory effect to the growth of Staphylococcus aureus, Staphylococci, and Pseudomonas aeruginosa. The Bitoon seeds were extracted for the purpose of Phytochemical analysis. Bitoon seeds were collected and shredded. Six treatments were prepared with three replicates each and applied to cultured bacteria which are Staphylococcus aureus, Staphylococci, and Pseudomonas aeruginosa,. The treatments include 100%, 75%, 50%, 25% and the controlled substances Chloramphenicol for Positive control and Pure Distilled water for Negative control. Antibacterial assay was performed using paper disc diffusion method. Zone of inhabitation were measured and compared among treatments means. Based on the result, the chemical was in extract concentration because they are steroidal that reduce the virulence or pathogenicity of cells in the body. Therefore, the seeds contain certain chemicals such as alkaloids, flavonoids, saponins, tannins, steroids, and anthraxquinone that can reduce bacteria. ANOVA revealed that there is a significant inhibitory effect of Bitoon seeds extract on the growth of Staphylococcus aureus, Staphylococci, and Pseudomonas aeruginosa. Hence, Bitoon seeds (Barringtonia asiatica) extract can be an alternative to commercial antibiotic in treating external infection caused by bacteria.
ACKNOWLEDGEMENT
Beyond any doubts, research has lead people, with undying efforts; arrive with facts and data that become more meaningful in total course of discovering new insights. With the
An Investigatory Project Submitted As Entry to the
2010 Regional Science Fair
Jorge Andrew C. Bagaipo
Jessa Mae C. Macana
Loriel Mari E. Berte
Researchers
Ma. Edylyn L. Noguerra
Adviser
ABSTRACT
The study entitles “Bitoon seeds Extract (Barringtonia asiatica) as Potential Antibacterial Agent aims that the antibacterial agent of the seeds and determines its inhibitory effect to the growth of Staphylococcus aureus, Staphylococci, and Pseudomonas aeruginosa. The Bitoon seeds were extracted for the purpose of Phytochemical analysis. Bitoon seeds were collected and shredded. Six treatments were prepared with three replicates each and applied to cultured bacteria which are Staphylococcus aureus, Staphylococci, and Pseudomonas aeruginosa,. The treatments include 100%, 75%, 50%, 25% and the controlled substances Chloramphenicol for Positive control and Pure Distilled water for Negative control. Antibacterial assay was performed using paper disc diffusion method. Zone of inhabitation were measured and compared among treatments means. Based on the result, the chemical was in extract concentration because they are steroidal that reduce the virulence or pathogenicity of cells in the body. Therefore, the seeds contain certain chemicals such as alkaloids, flavonoids, saponins, tannins, steroids, and anthraxquinone that can reduce bacteria. ANOVA revealed that there is a significant inhibitory effect of Bitoon seeds extract on the growth of Staphylococcus aureus, Staphylococci, and Pseudomonas aeruginosa. Hence, Bitoon seeds (Barringtonia asiatica) extract can be an alternative to commercial antibiotic in treating external infection caused by bacteria.
ACKNOWLEDGEMENT
Beyond any doubts, research has lead people, with undying efforts; arrive with facts and data that become more meaningful in total course of discovering new insights. With the