Preview

Sputum Report

Good Essays
Open Document
Open Document
601 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Sputum Report
Discussion After doing a series of different biochemical tests for the isolated organisim from the sputum sample, it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC, SBAP, CHOC and also a direct gram stain of the sample was performed. After a day of incubation, the MAC agar gave a negative result, therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth of colonies. The CHOC agar had a mixed formation of colonies (gray colonies and 2+ tan colonies), so it was isolated in another CHOC agar and it was mixed again. A gram stain procedure was done and it showed both Gram positive and negative cocci. …show more content…
Each was isolated on different plates to have a primary culture, SBAP 1 and SBAP 2. In SBAP 1, a split plate was done, by inoculating the B hemolytic colonies and the tan colonies from the CHOC. It gave a mixed growth, so the tan colonies were inoculated to SBAP 2 in order to isolate the tan colonies without being mixed with the other colony. In SBAP 2, the split plate of γ-hemolytic colonies and tan colonies from the CHOC. After incubation, the tan colonies showed irregular colony morphology which has a formation of halo clearing on the sides of the …show more content…
This hemolysis is attributed to toxins formed by Group A streptococci called streptolysins. Streptolysins can destroy not only red blood cells, but also the white blood cells responsible for fighting off bacteria and disease, as well as other body cells[2]. The cause of strep throat is bacteria known as Streptococcus pyogenes, also known as group A streptococcus. Streptococcal bacteria are highly contagious. They can spread through airborne droplets when someone with the infection coughs or sneezes, or through shared food or drinks. You can also pick up the bacteria from a doorknob or other surface and transfer them to your nose, mouth or

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Lab Report On Unknown 32

    • 591 Words
    • 3 Pages

    The first test conducted on unknown bacteria 32 was the Gram stain. From this stain, unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria, Gram-positive rods and Gram-positive spirillium. Next, the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green, steam, and safranin it was found that unknown bacteria 32 did not contain endospores.…

    • 591 Words
    • 3 Pages
    Satisfactory Essays
  • Powerful Essays

    Double Unknown Lab Report

    • 1229 Words
    • 5 Pages

    The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus aureus with a positive confirmatory test, mannitol salt agar, showing consistent results as well for S. aureus. The Gram-negative bacteria were Pseudomonas aeruginosa with a positive confirmatory…

    • 1229 Words
    • 5 Pages
    Powerful Essays
  • Powerful Essays

    The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and, through a series of metabolic tests, identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45, henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment.…

    • 1738 Words
    • 7 Pages
    Powerful Essays
  • Better Essays

    Unknown Lab Report

    • 2145 Words
    • 9 Pages

    The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and sucrose fermentation, Kugler/iron agar, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, bile esculin, indole, urease, DNase, and coagulase.…

    • 2145 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Unknown Microorganism

    • 1335 Words
    • 6 Pages

    The streak for isolation was chosen to see the formation of the different colonies and to see if the bacteria would cause growth on the plate. After observing the plate, it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative, other bacteria’s were able to be eliminated from the options. The Triple Sugar Iron Test was chosen next because the unknown could be tested for glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction. If the organism is able to ferment glucose and lactose and/or sucrose it will turn the medium yellow throughout (Leboffe & Burton 329). When the Triple Sugar Iron Test was complete, it showed a yellow butt, yellow slant and gas production. This determined that the unknown ferments glucose and one or both of the other sugars. The gas was produced by fermentation. The color change in the agar brought the unknown down to two options of unknowns, Escherichia coli or Hafnia alvei. The SIM medium test was chosen next to see sulfur reduction, indole production from tryptophan…

    • 1335 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Organism Project

    • 615 Words
    • 3 Pages

    Whole colony appearance: CircularMargin (Edge): EntireElevation: Flat and raisedGrowth in liquid medium:Pattern of growth on media: BeadedSize: SmallTexture: RoughAppearance on routine isolation media: DullAppearance on special media: DullOptical Property: Opaque…

    • 615 Words
    • 3 Pages
    Good Essays
  • Good Essays

    After the gram stain test I carried out a catalase test. This test was carried out to investigate if my unknown was streptococci (negative) or staphylococci (positive) and or micrococci (positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide.…

    • 589 Words
    • 3 Pages
    Good Essays
  • Better Essays

    The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique is when a cultures has multiple species they are able to be more easily identified once they have been isolated. This experiment is much like the experiments completed on an agar plate but on one a much larger scale and where techniques can be perfected before the use of agar plates and specimens are used. Because this experiment uses materials that represent those used in the streaking on agar plates the ability to simulate the events that occur when streaking is similar and allows for visualization instantly.…

    • 1538 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Strep throat is a bacterial infection that’s causes the back of the throat to be sure, scratchy, red, and inflamed in the human body. Most people develop symptoms of swollen lymph nodes, fever, swollen tonsils, headache, chills, and loss of appetite, nausea, vomiting, and the little white pus pocket in the back of the throat.…

    • 673 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Strep throat is a very contagious disease that is most common among teenagers. This disease is caused by infection with group A streptococci bacteria. It can be spread among humans in multiple ways. For example, the first page of the packet titled "Strep Throat" explains that strep can be spread by simply sneezing, coughing, shaking hands, and hanging around large groups of people. The first page of the packet titled "Strep Throat" also explains the symptoms of this contagious disease. For instance, symptoms include red and white patches in the throat, trouble swallowing, tender and swollen glands ( lymph nodes ) on the sides of your neck, red and big tonsils, headache, stomach pain, fever, feeling weak or sick, loss of appetite, and…

    • 242 Words
    • 1 Page
    Good Essays
  • Good Essays

    Strep Throat

    • 923 Words
    • 3 Pages

    “Strep throat is a bacterial infection that is caused by streptococcal (strep) bacteria. It is found in the throat and on the tonsils.” (WebMd, 2013) When you have strep throat your throat becomes irritated and inflamed which causes a sudden, severe sore throat. There are many kinds of strep bacteria and some cause a more serious illness than others. It is common for people to think they have strep when they have a sore throat but most sore throats are caused by viral infections. However, a sore throat caused by a viral infection can be just as painful as strep throat.…

    • 923 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Streptococcus was first discovered in 1874 by Theodor Billroth. However, that scientist at that time didn’t know there were many types of strep bacteria. In 1883, Fehleisen discovered the bacteria that causes strep throat. Finally, in 1918, Rebecca Lancefield started breaking up strep into different categories. The category that strep throat belongs to is called Streptococcus pyogenes. Therefore, Streptococcus pyogenes is the bacteria that causes strep throat. In 2002, there was a large outbreak of strep throat at the Children’s Hospital in Pittsburg. There were over 300 people who were diagnosed with strep throat. Also, some of the people had rheumatic fever and other strep related infections. This was a serious outbreak because the bacteria…

    • 192 Words
    • 1 Page
    Good Essays
  • Better Essays

    Gram Staining

    • 1268 Words
    • 6 Pages

    A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923, Gram-positive cocci, in purple) andEscherichia coli (Escherichia coli ATCC 11775, Gram-negative bacilli, in red), the most common Gram stain reference bacteria…

    • 1268 Words
    • 6 Pages
    Better Essays
  • Good Essays

    Streptococci are Gram positive cocci (spherical or ovoid) that most of the time occur in pairs and chains. Streptococci are facultatively anaerobic and catalase negative (Hardie, 1986). Carbohydrates are being metabolized fermentatively; lactic acid is the vital metabolite. Streptococci yield the enzyme leucine aminopeptidase (LAP), this is also called leucine arylamidase (SMI,…

    • 738 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Kefir Coculture Essay

    • 2474 Words
    • 10 Pages

    The following microbiological analyses were performed: (i) determination of total aerobic counts on nutrient agar (Oxoid) at 30°C for 48 h, (ii) coliforms on MacConkey agar (Oxoid) by incubation at 30°C for 24 h, (iii) yeasts and molds by incubation on malt agar (Oxoid) (pH adjusted to 4.5 by sterile solution of 10% lactic acid) at 30°C for 48 h, and (iv) staphylococci by incubation on Staphylococcus agar No. 110 medium (Oxoid) at 37°C for 48h [Atlas,…

    • 2474 Words
    • 10 Pages
    Better Essays

Related Topics