tissue culture
CALLUS INDUCTION
By: Sonali Pradhan,08-11-2013
ABSTRACT
The present study describes a reliable method for callus induction of Hybanthus ennaspermus,an important medicinal plant plant. Calli were in vitro initiated from leaves segments using Murashige and Skog (1962) basal media. The effect of NAA (_-naphthalene acetic acid) and 2,4-D (2,4-Dichlorophenoxy acetic acid) on callus induction was investigated. It was found that growth regulators and their concentrations have obvious effects on the callus induction, the increment of callus index and callus physical appearance. The best callus induction capabilities were obtained with low levels of NAA However, supplemented medium with high concentrations of NAA led to relatively low callus frequency and the callus obtained turned to brown color. Generally, the highest frequencies of callus formation from leaves, explants were observed in the medium containing 1.0 mg /l of NAA..
INTRODUCTION:
Medicinal plants, which form the backbone of traditional medicine, have in the last few decades been the subject for very intense pharmacological studies; this has been brought about by the acknowledgement of the value of medicinal plants as potential sources of new compounds of therapeutics value and as sources of lead compounds in the drug development. In developing countries, it is estimated that about 80% of the population rely on traditional medicine for their primary health care. There arises a need therefore to screen medicinal plants for bioactive compounds as a basis for further pharmacological studies.
Hybanthus enneaspermus L. Muell, a member of the Violaceae family, is a rare, perennial herb found in some of the warmer parts of the Deccan peninsula in India. Popularly called ‘Ratanpurus’ by the local Yanadi and Santal tribes, villagers and herbalists, this ethnobotanical herb is known to have unique medicinal properties. The preparations made
By: Sonali Pradhan,08-11-2013
ABSTRACT
The present study describes a reliable method for callus induction of Hybanthus ennaspermus,an important medicinal plant plant. Calli were in vitro initiated from leaves segments using Murashige and Skog (1962) basal media. The effect of NAA (_-naphthalene acetic acid) and 2,4-D (2,4-Dichlorophenoxy acetic acid) on callus induction was investigated. It was found that growth regulators and their concentrations have obvious effects on the callus induction, the increment of callus index and callus physical appearance. The best callus induction capabilities were obtained with low levels of NAA However, supplemented medium with high concentrations of NAA led to relatively low callus frequency and the callus obtained turned to brown color. Generally, the highest frequencies of callus formation from leaves, explants were observed in the medium containing 1.0 mg /l of NAA..
INTRODUCTION:
Medicinal plants, which form the backbone of traditional medicine, have in the last few decades been the subject for very intense pharmacological studies; this has been brought about by the acknowledgement of the value of medicinal plants as potential sources of new compounds of therapeutics value and as sources of lead compounds in the drug development. In developing countries, it is estimated that about 80% of the population rely on traditional medicine for their primary health care. There arises a need therefore to screen medicinal plants for bioactive compounds as a basis for further pharmacological studies.
Hybanthus enneaspermus L. Muell, a member of the Violaceae family, is a rare, perennial herb found in some of the warmer parts of the Deccan peninsula in India. Popularly called ‘Ratanpurus’ by the local Yanadi and Santal tribes, villagers and herbalists, this ethnobotanical herb is known to have unique medicinal properties. The preparations made
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