Coomassie Blue G-250 which is red brown colour in acidic solution. When the protein binds to the dye‚ the pKa of the dye shifts and causes the dye to turn blue. The maximum absorbance spectrum of the dye is at 595nm. According to Beer-Lambert Law‚ a standard curve of absorbance versus protein concentration produced by measuring the absorbance of the protein solutions of a known concentration is often used to estimate the protein concentration of an unknown solution. Other than that‚ the Bradford protein assay
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spectrophotometer to determine the absorbance‚ it is clearly seen that the concentration and the absorbance are directly proportional to one another. This therefore‚ served as a guide in order to find out if the unknown sample being tested follows the given standard. A standard curve plot is also drawn‚ plotting A595 versus the conc. of BSA to be able to tell the unknown concentration of the sample. [1] In conclusion‚ the unknown protein solutions’ concentration and absorbance were both directly proportional
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02)mL/100(±0.08)mL]*[0.00020808(±0.0000005)mol/L] = 0.000062424(±0.2807%) = 0.000062424(±0.0000002) 5. From the Calibration curve of Absorbance Vs Concentration‚ we know the equation of the graph is : y = 10553.63(±190.5558)x - 0.00363(±0.007721) Where‚ y is the absorbance and x is the concentration. We know the absorbance of the unknown is 0.096. Therefore‚ 0.096 = 10553.63(±190.5558)x - 0.00363(±0.007721) x= [0.096+0.00363(±0.007721)]/ [10553.63(±190.5558)] = 0
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– Thiocyanate System through the use of a UV-Vis Spectrophotometer. It is achieved by inserting a series of solutions with varying concentrations of Fe3+ and SCN- into a cuvette‚ then to the spectrophotometer in order to determine the degree of absorbance of the [FeSCN2+].First‚ standards solutions are used in order to determine several critical values. Then‚ unknown solutions are used in order to determine their equilibrium constant. Results from the experiment showed that the average equilibrium
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Experiment #10 The Chemistry of Natural Waters Lab Report Jake Shoemaker Group: 11/5/10 Chemistry 111 Section 103 Shoemaker 1 Introduction: Water hardness is defined as a measure of the amount of calcium and magnesium salts dissolved in water4. Hard water is water that contains large amounts of dissolved calcium and magnesium cations‚ and soft water is water that little or none of the cations10. The hardness of water is important because it affects numerous aspects of our life. Water hardness
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Synthesis and Analysis of the Coordination Compound Pentaaminechlorideocobalt (III) Chloride Heaven Lin and Forest Lin W.M. Keck Science Department‚ Claremont McKenna‚ Pitzer‚ and Scripps Colleges 925 N. Mills Avenue‚ Claremont‚ California 91711 Abstract Through using the spectrometer and titrations‚ the amount of the coordination compound‚ pentaaminechloridocobalt (III) chloride‚ was determined through a synthesis through an oxidation reduction of cobalt (II) chloride hexahydrate (CoCl2·6H2O)
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demonstrated that the color of a given food dye depended on which light was transmitted in the visible spectrum and which light was absorbed. A quantitative measurement of the absorbance spectrum of each food dye was obtained through spectrophotometry.1 Through this method‚ we determined that the wavelength of maximum absorbance of a given food dye was directly correlated to the color of that food dye. Further‚ darker food dyes absorbed more light than lighter food dyes (Table 1). The relationship
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Determining the concentration of red dye #40 in an unknown solution Introduction: White light is composed of many different wavelengths of light combined together. A spectrophotometer is an instrument that shines a single wavelength of light of a known intensity into a solution and then measures the intensity of the light exiting the solution. If a solution contains
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pH Titration of Phosphoric Acid & Spectrophotometer Determination of the Percentage of Phosphoric Acid‚ in Cola Drinks (Experiment 23 & 24) Robbie Kinsey Partner: Debnil Chowdhury Chem. 1312-D TA’s: Russell Dondero & Sylvester Mosley Performed Feb. 16‚2000 Purpose The objective of this lab was to determine the concentration of phosphoric acid in cola through two different methods: a pH titration and a spectrophotometer
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In tube 2‚ there is a decrease in the absorption rate when the reaction mix is mixed. In test tube 3‚ the readings of the absorption rate decreases when in the presence of light. There is a decrease in reading up till the 60th minute and then the absorbance rate decreases in test tube 4. In test tube 5‚ there is a decrease in absorption rate when DCMU is mixed. The readings for the absorption rate increases up till the 30th minute then it would decrease in test tube 6. In test tube 7‚ the readings
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