Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
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equilibrium constant for the formation of an iron(III) thiocyanate complex ion (FeSCN2+) from Fe3+ and SCN- . The quantitative preparation of several solutions and subsequent measurement of the solution absorbance using a spectrophotometer are the techniques that will be used in this experiment. The absorbance measurement gives the concentration of FeSCN2+. The concentrations of Fe3+ and SCN- are obtained as the difference between the initial concentration and the concentration consumed by the formation
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test tube (containing all components except iron solution) was then added to the spectrophotometer and set to 0 absorbance/100% transmittance. The absorbance of each solution was measured at 510 nm quickly after they were mixed. The table on the next page shows the absorbance of each test tube along with the micrograms of iron they contain. Test Tube | Micrograms of iron | Absorbance (nm) | 1 | 0.0 (blank) | 0 nm | 2 | 10 | 0.20
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Objectives 1 1. To prepare a standard curve of absorbance versus protein concentration by using Bovine Serum Albumin (BSA). 2. To determine BSA concentration in two sample solutions. 3. To determine protein concentration in apple juice. Introduction What is Bradford protein assay? The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution [1]. There are several reagents that can be used to determine the concentration
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adenosine triphosphate‚ also known as ATP. In order to analyze unknown glucose levels‚ a DNS assay was performed. By using 2-hydroxy-3‚5-dinitrobenzoic acid to oxidize the aldehyde group on the carbohydrate‚ the reducing end of glucose increases in absorbance of 540 nm. Using a UV spectrophotometer‚ the concentration was calculated by using a regression line of the standard curve. The experimental data concluded a 1068.912 µg/ml. With the actual concentration being 1200.000 µg/ml‚ a 10.924% error
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Introduction: Enzymes are a substance produced by a living organism that acts as a catalyst to activate a specific reaction. The purpose of this experiment was to figure out if the temperature of the reaction would rise‚ will the absorption rise as well. Reactions use energy‚ If there is energy than heat occurs. The Hypothesis that was figured out was‚ If the temperature rises‚ then the absorption will also go up. The Independent variable that was tested was temperature. The dependent variable that
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Cell Lab Report Experiments : 1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods.
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Plot absorbance (vertical axis) against concentration of p‐nitrophenol (horizontal axis). Determination of Activation Energy of the Reaction Determination of Activation Energy of the Reaction Add 0.6 mL of enzyme solution to the reaction tubes and mix Determination of Activation Energy of the Reaction Add 0.6 mL of enzyme solution to the reaction tubes and mix Take 1 mL samples at given time points & add to tubes containing 2 mL of 0.05 M NaOH Measure absorbance against calibration blank from the calibration curve
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sample was used as a blank to set the zero absorbance for the spectrophotometer. The direction of the test tube was marked and noted for future use. The student then selected a New Absorbance vs. Concentration experiment in SpectroPro. This was done in order to calibrate the spectrophotometer. The wavelength setting was set on the spectrophotometer at 620nm and the machine was set absorbance‚ listed as unit (A). The student set the blank to zero absorbance and finished the calibration of the spectrophotometer
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1 2 3 4 5 Concentration (mg/ml) 0.2 0.04 0.008 0.0016 0.00032 Using UV-Spectrophotometer absorbance was taken above all the solution for flupentixol dihydrochloride (at 229 nm wavelength) and for melitracen hydrochloride (at 258 nm wavelength). The observed value was plotted against concentration and a linear regression equation was obtained
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