intensity experiment‚ our first set of data illustrated strange correlations between the three tubes placed in front of a light source‚ so the data provided in e-campus was used. The tube that was 24 cm away from the light had the greatest decrease in absorbance over the course of 20 minutes‚ followed by the 30 cm and then the 49 cm light apparatus. The tube that was placed in the dark (wrapped in foil) remained a dark blueish-green color whereas the tubes that were exposed to the light became a lighter
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reactive. Cu2+ is reduced to Cu1+ by protein in alkaline solution and the purple color change is the result of two molecules of BCA chelating to cuprous ion (2). This makes the reducing agents create a cuprous ion to chelate with the BCA‚ increase the absorbance readings‚ and skew the accuracy of the protein concentration. BCA does not react to contaminants such as detergents (2). In the Bradford assay in the readings are usually caused by elevated levels of detergent (3). When Coomassie Brilliant Blue
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experiment the rate of isomerism for the coordination complex cis[Co(en)2Cl2]Cl was determined using UV/Vis spectrometry. Using a range of wavelengths the optimum spectrometer setting for analysis was identified. The corresponding maximum and minimum absorbance of the cis and trans isomers respectively at 540 nm meant that it was selected as the wavelength to determine the rate of conversion between the isomers. The first order rate constant was calculated to be 0.0092 m-1 which is somewhat similar to
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NAME: EDWARD AFUTU INDEX NO: 8030212 EXPERIMENT: I.2.2.2. DEMONSTRATOR: MISS NAOMI KABIRI
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concentration or ppm can be measured using the absorbance of the solution and comparing it to a standard curve for calcium². Whereas magnesium‚ needs to be determined using the standard addition method. The concentrations of each respective molecule can be presented as CaO and MgO percentages of the unknown limestone sample. Materials and Methods The compound calcium carbonate (CaCO3‚ CAS: 471-34-1‚ 204.89ppm) was utilized to compare its absorbance to the unknown sample of limestone # 8066 on
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Isolation and Characterization of Maize Acid Phosphatase | BCHM 405 | Aaron BANESEH 10307275 | Table of Contents 1 Table of Contents 2 2 Introduction 4 3 Experimental Procedures 5 3.1 Sample preparation 5 3.2 Initial Extraction of Acid Phosphatase 5 3.3 Determination of Protein Concentration by the Folin-Lowry Assay 5 3.4 Specific Activity Assay 6 3.4.1 Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of
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hypothesized and tested within the confines of the experiment on their effects on the productivity of lactase enzyme. The measurement of light absorbance levels of different solutions based on both the concentration of o-nitrophenol (ONP) as well as the pH of the solution tested. To begin with‚ differing concentrations of ONP were tested to find the absorbance levels of ONP. Three different pH values‚ 5‚ 6‚ and 7‚ in solution‚ were tested over a period of 8 minutes and their absorbance’s recorded. Both
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Hand Soap Effectiveness on Escherichia Coli Abstract: Bacteria are an essential part of many different living organisms and ecosystems across the globe. Without bacteria there would have never had been life in the first place. But some bacteria have evolved to cause to humans‚ resulting in the need to eliminate them through such methods as soap. Hand soap is a crucial part of human health‚ especially in such places as schools and hospitals. In this experiment I wanted to determine the effectiveness
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Fe3+ +SCN- ⇔ FeSCN2+ The concentrations of the three substances at equilibrium will be determined by the stoichiometry of the reaction and the stoichiometric determination of the concentration of the complex AB. Beer’s Law tells us that the absorbance‚ A‚ is proportional to the path length‚ l‚ and the molar concentration‚ c: A= e lc The proportionality constant‚ ‚ is called the molar extinction coefficient‚ e. For the spectrometer to be used‚ the path length‚ ‚ is 1.00cm. At the wavelength
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by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount of light absorbed by the solution (absorbance) at various wavelengths. The preparation of the extraction included the use of a scale to weigh samples‚ the operation
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